Hostname: page-component-848d4c4894-8bljj Total loading time: 0 Render date: 2024-06-19T10:26:16.605Z Has data issue: false hasContentIssue false
  • English
  • Français

Histochemical localization of enzymes of various metabolic pathways in the testes of buffaloes, goats and rams

Published online by Cambridge University Press:  27 March 2009

G. S. Bilaspuri  and
S. S. Guraya
G. S. Bilaspuri
Affiliation:
Reproductive Biology Laboratory, Department of Biology, Guru Nanak Dev University, Amritsar-143005, Punjab, India
S. S. Guraya
Affiliation:
Reproductive Biology Laboratory, Department of Biology, Guru Nanak Dev University, Amritsar-143005, Punjab, India
Get access Rights & Permissions [Opens in a new window]

Summary

Isocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), glutamate dehydrogenase (GDH), β-hydroxybutyrate dehydrogenase (β-OH-BDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) were histochemically located in the testes of buffaloes, goats and rams. The enzyme activities varied with the enzyme, species and cell type. The activities in the seminiferous tubules were correlated with the stages of seminiferous epithelial cycle (SEC). During this cycle, the activities in the Sertoli cells, spermatogonia and spermatocytes remained unaltered in contrast to those in the spermatids. The activities of SDH, ICDH and MDH were relatively greater in buffalo, while goat and ram resembled each other quite closely. ICDH and MDH preferred NADP to NAD. In the three species, the activities of ICDH, SDH and MDH generally followed an increasing order. G-6-PDH was greater in the interstitial tissue of buffalo than in goat and ram; the maximum activity of this enzyme in each species was found in the spermatogonia. In comparison with G-6-PDH, GDH was less evident in the interstitial tissue of buffalo and goat; Sertoli cells and spermatogonia also showed relatively less MDH activity whereas the other germ cells may have relatively less, similar or more, GDH activity depending on the species. β-OHBDH activity was similar in the interstitial tissue of the three species, but in the seminiferous tubule, the activity was less in goat. But for GDH and β-OH-BDH which could show different results, the activities of other enzymes generally decreased from spermatogonia through spermatocytes to spermatids but increased during spermiogenesis. In spermatozoa, the enzymes were observed only in the mid-piece. The possible physiological significance of the results is discussed in relation to different metabolic pathways.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 102 , Issue 2 , April 1984 , pp. 269 - 274
Copyright
Copyright © Cambridge University Press 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ambadkar, P. M. & George, J. C. (1964). Histochemical localization of certain oxidative enzymes in rat testis. Journal of Histochemistry and Cytochemistry 12, 587–592.CrossRefGoogle ScholarPubMed
Bilaspuri, G. S. (1978). Morphological, histochemical and biochemical studies on the testes of some farm animals. Ph.D. thesis, Punjab Agricultural University, Ludhiana, India.Google Scholar
Bilaspuri, G. S. & Guraya, S. S. (1978). Comparative histochemical localization of steroid dehydrogenases in the testis of buffalo, goat and ram. Vth International Congress on Hormonal Steroids, New Dehli.Google Scholar
Bilaspuri, G. S. & Guraya, S. S. (1980 a). Quantitative studies on spermatogenesis in buffalo (Bubalus bubalis). Reproduction, Nutrition, Développement 20, 975–982.CrossRefGoogle ScholarPubMed
Bilaspuri, G. S. & Guraya, S. S. (1980 b). Quantitative studies on seasonal variations in testicular lipids of goat (Capra hircus) testes. Indian Journal of Experimental Biology 18, 205–206.Google ScholarPubMed
Bilaspuri, G. S. & Guraya, S. S. (1981). Comparative lipid composition in the testes of buffalo, goat and ram. Indian Journal of Animal Sciences 51, 1038–1044.Google Scholar
Bilaspuri, G. S. & Guraya, S. S. (1982). Distribution of oxidases in the testes of buffalo, goat and ram: an histochemical study. Reproduction, Nutrition, Développemt. 22, 505–510.CrossRefGoogle Scholar
Bilaspuri, G. S. & Guraya, S. S. (1983 a). Histochemical localization of phosphatases in the testes of buffalo, goat and ram. Indian Journal of Animal Sciences 53, 605–608.Google Scholar
Bilaspuri, G. S. & Guraya, S. S. (1983 b). Histochemical localization of glycolytic enzymes, alcoholand secondary alcohol dehydrogenases in the testes of buffaloes, goats and rams. Journal of Agricultural Science, Cambridge 101, 457–462.CrossRefGoogle Scholar
Blackshaw, A. W. (1970). Histochemical localization of testicular enzymes. In The Testis vol. II (ed. Johnson, A. D., Gomes, W. R. and Van Demark, N. L.), pp. 73–123. New York: Academic Press.CrossRefGoogle Scholar
Blackshaw, A. W., Hamilton, D. & Massey, P. F. (1973). Effect of scrotal heating on testicular enzymes and spermatogenesis in the rat. Australian Journal of Biological Sciences 26, 1395–1407.CrossRefGoogle ScholarPubMed
Blackshaw, A. W. & Samisoni, J. I. (1967 a). Histochemical localization of some dehydrogenase enzymes in the bull testis and epididymis. Journal of Dairy Science 50, 747–752.CrossRefGoogle ScholarPubMed
Blackshaw, A. W. & Samisoni, J. I. (1967 b). The testis of cryptorchid ram. Research in Veterinary Science 8, 187–194.CrossRefGoogle ScholarPubMed
Chayen, J., Bitensky, L. & Butcher, R. G. (1973). Practical Histochemistry. New York: John Wiley and Sons.Google Scholar
Chung, K. W. (1974). A morphological and histochemical study of Sertoli cells in normal and XX sexreversed mice. American Journal of Anatomy 139, 369–388.CrossRefGoogle Scholar
Free, M. J. (1970). Carbohydrate metabolism in the testis. In The Testis vol. II (ed. Johnson, A. D., Gomes, W. R. and Van Demark, N. L.), pp. 125–192. New York: Academic Press.Google Scholar
Guraya, S. S. & Bilaspuri, G. S. (1976). Stages of seminiferous epithelial cycle and relative duration of spermatogenic processes in buffalo. Gegenbaurs Morphologisches Jahrbuch Leipzig 122, 147–161.Google ScholarPubMed
Hitzeman, J. W. (1962). Development of enzyme activity in the Leydig Cells of the mouse testis. Anatomical Record 143, 351–362.CrossRefGoogle ScholarPubMed
Ito, M. (1966). Histochemical observations of oxidative enzymes in irradiated testis and epididymis. Radiation Research 28, 266–277.CrossRefGoogle ScholarPubMed
Koudstaal, J., Frensdorf, E. L., Kremer, J., Mudde, J. M. & Hardonk, M. J. (1967). The histochemical pattern of human adult testis. Acta Endocrinologica 55, 415–426.Google Scholar
Lehninger, A. L. (1975). Biochemistry. The Molecular Basis of Cell Structure and Function. New York: Worth Pub. Inc.Google Scholar
Livni, N. & Yaffe, H. (1974). Histochemistry of normal and ethionine treated rat testis. Histochemistry 40, 329–341.CrossRefGoogle Scholar
Pearse, A. G. E. (1960). Histochemistry, Theoretical and Applied. 2nd edn.London: J. A. Churchill.Google Scholar
Posalaky, Z. (1965). Activity of different dehydrogenases and diaphorases in the spermatogenesis of the rat and its relation to motility. Acta Histochemica 20, 86–90.Google ScholarPubMed
Saidapur, S. K. (1976). Histochemical localization of Δ5-3β-, 17-β and 11 β-hydroxysteroid dehydrogenases and glucose-6-phosphate dehydrogenase activities in the testis of bat, Vesperugo pipestrellus (Dubson). Current Science 45, 729.Google Scholar
Samisoni, J. I. (1966). The effects of cryptorchidism on the growth and metabolic activity of testis. M.Sc. thesis, University of Queensland, Brisbane, Australia.Google Scholar