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Evaluation of cellulolytic exogenous enzyme-containing microbial inoculants as feed additives for ruminant rations composed of low-quality roughage

Published online by Cambridge University Press:  11 August 2020

M. S. Zayed Open the ORCID record for M. S. Zayed [Opens in a new window] ,
M. Szumacher-Strabel ,
D. A. A. El-Fattah Open the ORCID record for D. A. A. El-Fattah [Opens in a new window] ,
M. A. Madkour ,
M. Gogulski ,
V. Strompfová ,
A. Cieślak Open the ORCID record for A. Cieślak [Opens in a new window]  and
N. E. El-Bordeny
M. S. Zayed
Affiliation:
Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
M. Szumacher-Strabel
Affiliation:
Department of Animal Nutrition, Poznań University of Life Sciences, Poznań, Poland
D. A. A. El-Fattah
Affiliation:
Central Laboratory for Agriculture Climate, Agricultural Research Center, Dokki, Giza, Egypt
M. A. Madkour
Affiliation:
Department of Animal Nutrition, Animal Production Research Institute, Dokki, Giza, Egypt
M. Gogulski
Affiliation:
University Center for Veterinary Medicine, Poznań University of Life Sciences, Poznań, Poland Department of Preclinical Sciences and Infectious Diseases, Poznań University of Life Sciences, Poznań, Poland Centre of Biosciences SAS, Institute of Animal Physiology, Kosice, Slovakia
V. Strompfová
Affiliation:
Centre of Biosciences SAS, Institute of Animal Physiology, Kosice, Slovakia
A. Cieślak*
Affiliation:
Department of Animal Nutrition, Poznań University of Life Sciences, Poznań, Poland
N. E. El-Bordeny
Affiliation:
Department of Animal Production, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
*
Author for correspondence: A. Cieślak, E-mail: adam.cieslak@up.poznan.pl
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Abstract

The supplementation of ruminant diets with exogenous cellulolytic enzymes can improve their digestibility and feeding value. The objective of this study was to determine the effect of supplementing roughage (rice straw) and concentrate with inoculants containing four fungal strains (Pleurotus ostreatus, Phanerochaete chrysosporium, Trichoderma reesei and Trichoderma viride) and four bacterial strains (Paenibacillus polymyxa, Bacillus megaterium, Bacillus circulans and Bacillus subtilis), given separately or as a mixture, as a source of exogenous cellulolytic enzymes, on basic rumen parameters in vitro, including digestibility and methane production. A batch culture trial was used to select the best supplements, and a long-term rumen simulation technique (RUSITEC) was used to evaluate the effects of P. chrysosporium, B. subtilis, and a 1 : 1 mixture of these two on dietary component digestibility and fermentation parameters. In the batch culture evaluation, there were significant increases in the organic matter (OM) digestibility, the total gas production expressed as ml/g of dry matter, the OM, the neutral detergent fibre (NDF) and the acid detergent fibre (ADF) of the supplemented rations, as compared to the control, excluding the rations supplemented with T. viride and B. circulans. In the RUSITEC, the ration supplemented with mixed inoculants showed significantly higher digestibility of crude protein, ether extract, NDF and ADF than did the ration supplemented with the P. chrysosporium and B. subtilis inoculants. It can be concluded that the simultaneous use of fungal and bacterial exogenous cellulases on rice straw roughage improves its digestibility, without negative effects on other rumen parameters.

Keywords

In vitrorumenenzymeinoculantdigestibility
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 4 , May 2020 , pp. 326 - 338
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

Contributed equally to this study.

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