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The effect of urea and urea plus sodium sulphate on microbial protein production in the rumens of sheep given diets high in alkali-treated barley straw

Published online by Cambridge University Press:  27 March 2009

R. Elliott  and
D. G. Armstrong
R. Elliott
Affiliation:
Department of Agricultural Biochemistry and Nutrition, University of Newcastle upon Tyne, Newcastle upon Tyne, NEl 7RU
D. G. Armstrong
Affiliation:
Department of Agricultural Biochemistry and Nutrition, University of Newcastle upon Tyne, Newcastle upon Tyne, NEl 7RU
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Summary

The effects of virea and urea plus sodium sulphate on the production of microbial protein were studied in sheep fitted with rumen fistulae and re-entrant cannulae in the proximal duodenum and terminal ileum. Diets containing a high proportion (0·68–0·72) of sodium hydroxide-treated barley straw supplemented with starch, glucose and solka floe were fed at hourly intervals to the sheep at 830–870 g dry matter/24 h.

The addition of the urea and urea plus sulphate supplements, although inducing a stepwise reduction in overall digestibility of OM, resulted in increased microbial protein synthesis in the rumen, which was reflected in increased quantities of amino acid nitrogen entering the small intestine (8–38, 12–03 and 17–84 g of total amino acid nitrogen (TAA-N)/24h respectively). The efficiency of microbial protein production (g bacterial TAA-N/kg OM actually digested in the rumen) was significantly increased by the addition of urea and still further by additions of urea plus SO4-S (11·1, 20·2 and 29·6 grespectively).

Digestibilities of the individual amino acids in the small intestine were very similar on each dietary treatment. Apparent digestibilities of total amino acid-N within the small intestine (based on amounts entering the small intestine) were 0·623, 0·672 and 0·688 on the basal diet, basal diet plus urea and basal diet plus urea and S respectively.

The proportions of cyst(e)ine-S in rumen bacteria synthesized from the rumen sulphide pool were 0088, 0·075 and 0·669 on the basal diet, basal diet plus urea and basal diet plus urea and S respectively. The data confirm appreciable recycling of S into the rumen.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 99 , Issue 1 , August 1982 , pp. 51 - 60
Copyright
Copyright © Cambridge University Press 1982

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References

REFERENCES

Beever, D. E., Thomson, D. J. & Harbison, D. A. (1974). Energy and protein transformations in the rumen and the absorption of nutrients by sheep fed forage diets. In Proceedings of the 12th International Grassland Congress (ed. Oglivihov, V. G.), pp. 69–75. Moscow State Press.Google Scholar
Beever, D. E., Thomson, D. J., Pfeffer, E. & Armstrong, D. G. (1971). The effect of drying and ensiling grass on its digestion in sheep. Sites of energy and carbohydrate digestion. British Journal of Nutrition 26, 123–124.CrossRefGoogle ScholarPubMed
Belaich, J. P., Belaich, A. & Simonpietre, P. (1972). Uncoupling in bacterial growth: effect of pantothenate starvation on growth of Zymomonas mobilis. Journal of General Microbiology 70, 179–185.CrossRefGoogle Scholar
Bird, P. R. (1974). Sulphur metabolism and excretion studies in ruminants. XIII. Intake and utilization of wheat straw by sheep and cattle. Australian Journal of Agricultural Research 25, 631–642.CrossRefGoogle Scholar
Bird, P. R. & Fountain, R. D. (1970). Determination of sulphur in some biological materials. Analyst, London 95, 98–102.CrossRefGoogle ScholarPubMed
Bray, A. C. & Hemsley, J. A. (1969). Sulphur metabolism in sheep. IV. The effect of a varied dietary sulphur content on some body fluid sulphate levels and on the utilization of urea-supplemented roughage by sheep. Australian Journal of Agricultural Research1 20, 759–773.CrossRefGoogle Scholar
Brown, G. F., Armstrong, D. G. & Macrae, J. C. (1968). The establishment in one operation of a cannula into the rumen and re-entrant cannulae into the duodenum and ileum of the sheep. British Veterinary Journal 124, 78–82.CrossRefGoogle ScholarPubMed
Campling, R. C., Freer, M. & Balch, C. C. (1962). Factors affecting the voluntary intake of food by cows. 3. The effect of urea on the voluntary intake of oat straw. British Journal of Nutrition 16, 115–124.CrossRefGoogle ScholarPubMed
Coelho Da Sllva, J. F., Seeley, R. C., Thomson, D. J., Beever, D. E. & Armstrong, D. G. (1972). The effect in sheep of physical form on the sites of digestion of a dried lucerne diet. 2. Sites of nitrogen digestion. British Journal of Nutrition 28, 43–61.CrossRefGoogle Scholar
Corbett, J. L., Greenhaloh, J. F. D., Mcdonald, I. & Florence, E. (1960). Excretion of chromium sesquioxide administered as a component of paper to sheep. British Journal of Nutrition 14, 289–299.CrossRefGoogle Scholar
Donefer, E., Adeleye, I. O. A. & Jones, J. A. O. C. (1969). Effect of urea supplementation on the nutritive value of sodium hydroxide-treated oat straw. Advances in Chemistry Series 95, 328–342.CrossRefGoogle Scholar
Elliott, R. (1977). The significance of the dietary nitrogen: sulphur ratio in relation to mierobial protein synthesis in the rumens of adult sheep. Ph.D. thesis, University of Newcastle upon Tyne.Google Scholar
Forrest, W. W. & Walker, D. J. (1963). Calorimetric measurements of energy of maintenance of Streptococcus faecalis. Biochemical and Biophysical Research Communications 13, 217–222.CrossRefGoogle Scholar
Hogan, J. P. (1975). Protein and amino acid nutrition in the high producing cow. Quantitative aspects of nitrogen utilization in ruminants. Journal of Dairy Science 58, 1164–1177.CrossRefGoogle Scholar
Hogan, J. P. & Weston, R. H. (1967). The digestion of two diets of differing protein content but with similar capacities to sustain wool growth. Australian Journal of Agricultural Research 18, 973–981.CrossRefGoogle Scholar
Hume, I. D. (1970). Synthesis of mierobial protein in the rumen. III. The effect of dietary protein. Australian Journal of Agricultural Research 21, 305–314.CrossRefGoogle Scholar
Hume, I. D. & Bird, P. R. (1970). Synthesis of mierobial protein in the rumen. IV. The influence of the level and form of dietary sulphur. Australian Journal of Agricultural Research 21, 315–322.CrossRefGoogle Scholar
Hume, I. D., Moir, R. J. & Somers, M. (1970). Synthesis of mierobial protein in the rumen. 1. Influence of the level of nitrogen intake. Australian Journal of Agricultural Research 21, 283–296.CrossRefGoogle Scholar
Ishaque, M., Thomas, P. C. & Rook, J. A. F. (1971). Consequences to the host of changes in rumen mierobial activity. Nature New Biology 231, 253–256.CrossRefGoogle Scholar
Kennedy, P. M. & Siebert, B. D. (1972). The utilization of spear grass (Heteropogon contortus). II. The influence of sulphur on energy intake and rumen and blood parameters in cattle and sheep. Australian Journal of Agricultural Research 23, 45–56.CrossRefGoogle Scholar
Kennedy, P. M. & Siebert, B. D. (1973). The utilization of spear grass (Heteropogon contortus). III. The influence of the level of dietary sulphur on the utilization of spear grass by sheep. Australian Journal of Agricultural Research 24, 143–152.CrossRefGoogle Scholar
Klopfenstein, T. J. (1971). Enhancing forage value. Proceedings of the Minnesota Nutrition Conference, Minneapolis, pp. 27–35.Google Scholar
Mcmeniman, N. P., Ben-Ghedalia, D. & Elliott, R. (1976). Sulphur and cystine incorporation into rumen mierobial protein. British Journal of Nutrition 36, 571–574.CrossRefGoogle ScholarPubMed
MacRae, J. C. & Armstrong, D. G. (1969). Studies on intestinal digestion in the sheep. 1. The use of chromic oxide as an indigestible marker. British Journal of Nutrition 23, 15–23.CrossRefGoogle ScholarPubMed
Miller, E. L., Johnson, I. L., Briggs, M. C. E. & Kempsey, R. G. (1977). The effect of alkali and urea on ground and pelleted all-straw diets for sheep. Proceedings of the Nutrition Society 36, 129A.Google ScholarPubMed
Moir, R. J., Somers, M. & Bray, A. C. (1967–1968). Utilization of dietary sulphur and nitrogen by ruminants. Sulphur Institute Journal 3, 15–18.Google Scholar
Mowat, D. N., Ololade, B. G., Pheonix, L. & Bilansky, W. K. (1970). Increasing the availability of the energy in roughages by sodium hydroxide treatment. Proceedings of the University of Guelph Nutrition Conference, pp. 78–84.Google Scholar
Nolan, J. V. & Leng, R. (1972). Dynamic aspects of ammonia and urea metabolism in sheep. British Journal of Nutrition 27, 177–194.CrossRefGoogle ScholarPubMed
Ørskov, E. R. & Grubb, D. A. (1978). Validation of new systems for protein evaluation in ruminants by testing the effects of urea supplementation on intake and digestibility of straw with or without sodium hydroxide treatment. Journal of Agricultural Science, Cambridge 91, 483–486.CrossRefGoogle Scholar
Playne, M. J. & Kennedy, P. M. (1976). Ruminal volatile fatty acids and ammonia in cattle grazing dry tropical pastures. Journal of Agricultural Science, Cambridge 86, 367–372.CrossRefGoogle Scholar
Singh, M. & Jackson, M. G. (1971). The effect of different levels of sodium hydroxide spray treatment of wheat straw on consumption and digestibility by cattle. Journal of Agricultural Science, Cambridge 77, 6–10.CrossRefGoogle Scholar
Stevenson, A. E. & De Langen, H. (1960). Measurement of feed intake by grazing cattle and sheep. VII. Modified wet digestion method for determination of chromic oxide in faeces. New Zealand Journal of Agricultural Research 3, 314–319.CrossRefGoogle Scholar
Vercoe, J. E. (1969). The transfer of nitrogen from the blood to the rumen in cattle. Australian Journal of Agricultural Research 20, 191–197.CrossRefGoogle Scholar
Weston, R. H. & Hogan, J. P. (1968). Rumen ammonia in relation to characteristics of the diet and parameters of nitrogen metabolism. Proceedings of the Australian Society for Animal Production 7, 359–363.Google Scholar