Hostname: page-component-848d4c4894-xm8r8 Total loading time: 0 Render date: 2024-06-29T23:19:25.600Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of mixing ejaculates from different bulls on motility characteristics and acid production of the spermatozoa in a constant environment

Published online by Cambridge University Press:  27 March 2009

C. van Duijn
C. van Duijn
Affiliation:
Research Institute for Animal Husbandry ‘Schoonoord’, Hoogt 10, Utrecht, The Netherlands
Get access Rights & Permissions [Opens in a new window]

Extract

Motility characteristics of bull spermatozoa in mixed ejaculates of different bulls were compared with those of the unmixed samples after 40-to 50-fold dilution with clarified standard egg-yolk—citrate buffer (pH 6·80 ± 0·05 at 37° C., 6·75 ± 0·05 at 22–23° C).

Using the photo-electric equipment described by Rikmenspoel (1957b) and by Rikmenspoel & Van Herpen (1957) the mean velocity , the number of spermatozoa passing by a constant spot per time unit n, the total number of spermatozoa moving normally N, the velocity decrease with time ( — d/dt), the half-life period of the number of passages per time unit t½(n) and the half-life period of the total number of spermatozoa moving normally t½(N) were determined. For none of these characteristics was any statistical difference found between the actual results and the expected values based on calculation from the data obtained with the unmixed ejaculates.

Acid production was investigated by accurate determination of pH change on incubation of samples diluted ten times with standard egg-yolk—citrate buffer containing 736 mg. added fructose per 100 ml. Again there was no statistical difference between the values found with the mixed semen samples and the expected values.

It is concluded that differences observed by other authors must be due to the effect of environmental factors, i.e. to the semmal plasma, for which there is analogous evidence in human sperm physiology.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 55 , Issue 1 , August 1960 , pp. 69 - 74
Copyright
Copyright © Cambridge University Press 1960

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Campbell, R. C. & Jaffe, W. P. (1958). J. Agric. Sci. 50, 64.CrossRefGoogle Scholar
Dott, H. M. & Walton, A. (1958). J. Agric. Sci. 50, 267.CrossRefGoogle Scholar
Duijn, C. Van Jnr., & Rikmenspoel, R. (1960). J. Agric. Sci. 54, 300.CrossRefGoogle Scholar
Ehlers, M. H., Kushwaha, N. S. & Erb, L. E. (1958). J. Dairy Sci. 41, 1395.CrossRefGoogle Scholar
Frappell, J. P. & Williams, G. (1956). Proc. 3rd Int. Congr. Anim. Beprod. Cambridge.Google Scholar
Hess, E. A., Ludwick, T., Rickard, H. C. & Ely, F. (1954). J. Dairy Sci. 37, 649.CrossRefGoogle Scholar
Hess, E. A., Ludwick, T. M., Rickard, H. C. & Ely, F. (1958). Fertil. and Steril. 9, 239.CrossRefGoogle Scholar
Kušner, H. F. (1954). Izv. Akad. Nauk. SSSR (Ser. Biol.), no. 1, 32.Google Scholar
Rikmenspoel, R. (1957 a). Experientia, 13, 24.CrossRefGoogle Scholar
Rikmenspoel, R. (1957 b). Photoelectric and cinematographic measurements of the ‘motility’ of bull sperm cells. Thesis, Utrecht (The Netherlands).CrossRefGoogle Scholar
Rikmenspoel, R. & Van Herpen, G. (1957). Physics, Medicine & Biol. 2, 54.CrossRefGoogle Scholar
Rothschild, Lord (1949). Nature, Lond., 163, 358.CrossRefGoogle Scholar
Rozin, N. (1958). Acta med. or., Jerusalem, 17, 24.Google Scholar