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A colorimetric technique using chromium-ethylene diamine tetra acetate for measuring rumen volume

Published online by Cambridge University Press:  27 March 2009

K. El-Shazly ,
E. I. A. Ahmed ,
M. A. Naga  and
B. E. A. Borhami
K. El-Shazly
Affiliation:
Department of Animal Production, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt
E. I. A. Ahmed
Affiliation:
Department of Animal Production, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt
M. A. Naga
Affiliation:
Department of Animal Production, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt
B. E. A. Borhami
Affiliation:
Department of Animal Production, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt
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Summary

A method is described for the determination of rumen volume using Cr-EDTA colour measurement in a spectrophotometer at 550 nm. The results of several determinations of rumen volume comparing this method with Cr-determination as chromate and polyethylene glycol as markers gave similar values with differences ranging from 1 to 14% with an average of 6%. Direct measurements through evacuating rumen contents gave values 20·68% higher than those estimated by the colour method. The values were similar when corrected for dry matter.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 87 , Issue 2 , October 1976 , pp. 369 - 373
Copyright
Copyright © Cambridge University Press 1976

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