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Study of the relation between straw quality and its colonization by rumen micro-organisms

Published online by Cambridge University Press:  27 March 2009

Siva Bhat ,
R. J. Wallace  and
E. R. Ørskov
R. J. Wallace
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
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Summary

The relation between the dry-matter degradability (DMD) of different cultivars of barley straw and their colonization by rumen micro-organisms was studied in incubations of straw with the mixed population in situ and with pure cultures of rumen bacteria in vitro. The total attached population in situ was quantified by particle-associated i glutamate dehydrogenase (GDH) activity, while the cellulolytic population was assessed by bound carboxymethylcellulase (CMCase) activity. CMCase reached a peak between 24 and 36 h incubation, and the peak activity was highly correlated with DMD between 24 ) and 96 h. After 36 h, CMCase activity declined, particularly with the more degradable (varieties. GDH continued to increase after 36 h and had no relation to DMD. The adhesion of pure cultures of both cellulolytic and non-cellulolytic bacteria was measured i turbidimetrically in a 30 min incubation. No relation was found between DMD and the extent of adhesion by any of Ruminococcus flavefaciens, Bacteroides succinogenes, Butyrivibrio fibrisolvens or Selenomonas ruminantium. It was concluded the rate of degradation of straw is not determined by the initial rate of attachment of cellulolytic micro-organisms, but by the way in which colonization subsequently develops after attachment takes place

Type
Research Article
Information
The Journal of Agricultural Science , Volume 110 , Issue 3 , June 1988 , pp. 561 - 565
Copyright
Copyright © Cambridge University Press 1988

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References

Abrts, J. V., Debrander, D. L., Cottyn, B. G. & Buysse, F. X. (1977). Comparison of laboratory methods for predicting the organic matter digestibility of forages. Animal Feed Science and Technology 2, 337–349.Google Scholar
Agricultural Research Council (1980). The Nutrient Requirements of Ruminant Livestock. Farnham Royal: Commonwealth Agricultural Bureaux.Google Scholar
Akin, D. E. & Amos, W. E. (1975). Rumen bacterial degradation of forage cell walls investigated by electron microscopy. Applied Microbiology 29, 692–701.CrossRefGoogle ScholarPubMed
Akin, D. E. & Riosby, L. L. (1985). Degradation of Bermuda and orchard grass by species of rumen bacteria. Applied and Environmental Microbiology 50, 825–830.CrossRefGoogle Scholar
Cheng, K. J., Akin, D. E. & Costerton, J. W. (1977). Rumen bacteria: Interaction with particulate dietary components and response to dietary variations. Federation Proceedings 36, 193–197.Google Scholar
Dowman, M. G. & Collins, F. C. (1982). The use of enzymes to predict the digestibility of animal feeds. Journal of the Science of Food and Agriculture 33, 689–696.CrossRefGoogle Scholar
Groleau, D. & Forsbero, C. W. (1981). Cellulolytic activity of the rumen bacterium Bacteroides succinogenes. Canadian Journal of Microbiology 27, 517–530.CrossRefGoogle ScholarPubMed
Guggolz, J., Saunders, R. M., Kohler, G. o. & Klopfenstein, T. J. (1971). Enzymatic evaluation of processes for improving agricultural wastes for ruminant feeds. Journal of Animal Science 33, 167–170.CrossRefGoogle ScholarPubMed
Hartley, R. D., Deschard, G., Keene, A. S. & Mason, V. C. (1984). Changes in the chemical constitution of cereals and poor quality hay during upgrading. In Improvements in the Nutritive Value of Crops and Byproducts by Chemical or Biological Treatments. Proceedings of Second Seminar on the Upgrading of Crops and By-products, pp. 11–14. London: Ministry of Agriculture, Fisheries and Food.Google Scholar
Hartley, R. D., Jones, E. C. & Fenlon, J. S. (1974). Prediction of the digestibility of forages by treatment of their cell walls with cellulolytic enzymes. Journal of the Science of Food and Agriculture 25, 947–954.CrossRefGoogle Scholar
Hobson, P.N. (1969). Rumen bacteria. Methods in Microbiology 3B 133–149.CrossRefGoogle Scholar
Jones, D. I. H. & Hayward, M. V. (1973). A cellulase digestion technique for predicting the dry matter digestibility of grasses. Journal of the Science of Food, and Agriculture 24, 1419–1426.CrossRefGoogle ScholarPubMed
Kudo, H., Cheng, K. J. & Costerton, J. W. (1987). Electron microscopic study of the methylcellulosemediated detachment of cellulolytic rumen bacteria from cellulose fibers. Canadian Journal of Microbiology 33, 267–272.CrossRefGoogle ScholarPubMed
Latham, M. J., Brooker, B. E., Pettipher, G. L. & Harris, P. J. (1978). Adhesion of Bacteroides succinogenes in pure culture and in the presence of Ruminococcus flavefaciens to cell walls in leaves of perennial ryegrass (Lolium perenne). Applied and Environmental Microbiology 35, 1166–1175.CrossRefGoogle ScholarPubMed
Mcqueen, R. & Van Soest, P. J. (1975). Fungal cellulase and hemicellulase prediction of forage digestibility. Journal of Dairy Science 58, 1482–1491.CrossRefGoogle ScholarPubMed
Meers, J. L., Tempest, D. W. & Brown, C. M. (1970). ‘Glutamine (amide): 2-oxoglutarate aminotransferase oxido-reductase (NADP)’, an enzyme involved in the synthesis of glutamate by some bacteria. Journal of General Microbiology 64, 187–194.CrossRefGoogle ScholarPubMed
Nossal, N. G. & Heppel, L. A. (1966). The release of enzymes by osmotic shock from Escherichia coli in exponential phase. Journal of Biological Chemistry 241, 3055–3062.CrossRefGoogle ScholarPubMed
Silva, A. T., Wallace, R. J. & Okskov, E. R. (1987). Use of particle-bound microbial enzyme activity to predict the rate and extent of fibre degradation in the rumen. British Journal of Nutrition 57, 407–415.CrossRefGoogle ScholarPubMed
Tilley, J. M. A. & Terry, R. A. (1963). A two-stage technique for in vitro digestion of forage crops. Journal of the British Grassland Society 18, 104–111.CrossRefGoogle Scholar
Tuah, A. K., Lufadeju, E. & Orskov, E. R. (1986). Rumen degradation of straw. 1. Untreated and ammonia treated barley oat and wheat straw varieties and triticate straw. Animal Production 43, 261–269.Google Scholar
Van Soest, P. T. & Wine, R. H. (1967). Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell wall constituents. Journal of the Association of Official Agricultural Chemists 50, 50–55.Google Scholar