Hostname: page-component-848d4c4894-4hhp2 Total loading time: 0 Render date: 2024-04-30T10:18:05.641Z Has data issue: false hasContentIssue false
  • English
  • Français

Nitrogen digestion in forage-fed sheep with and without intraruminal propionate infusion

Published online by Cambridge University Press:  27 March 2009

C. J. Seal ,
D. S. Parker ,
J. Balcells  and
J. L. Mole
C. J. Seal
Affiliation:
Department of Biological and Nutritional Sciences, Faculty of Agriculture and Biological Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NEl 7RU, UK
D. S. Parker
Affiliation:
Department of Biological and Nutritional Sciences, Faculty of Agriculture and Biological Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NEl 7RU, UK
J. Balcells
Affiliation:
Department of Biological and Nutritional Sciences, Faculty of Agriculture and Biological Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NEl 7RU, UK
J. L. Mole
Affiliation:
Department of Biological and Nutritional Sciences, Faculty of Agriculture and Biological Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne NEl 7RU, UK
Get access Rights & Permissions [Opens in a new window]

Summary

Nitrogen balance and flow of nitrogen along the gastrointestinal tract were determined in growing wether sheep fed a dried grass pellet diet with or without intraruminal propionic acid infusion. Plasma free amino acid and glucose concentrations tended to be higher in peripheral blood of animals receiving propionate (P < 0·10). Overall total nitrogen and amino acid digestibilities and apparent digestibilities of each fraction along the digestive tract were not affected by infusion of propionic acid. The contribution of microbial amino acids to total amino acid flow in the duodenum measured using purine bases as the microbial marker averaged 0·49 and was not affected by propionic acid infusion. It is suggested that changes in circulating amino acid levels arise from reduced utilization of luminal amino acids by gastrointestinal tissues due to the increased availability of alternative energy-yielding substrates.

Type
Animals
Information
The Journal of Agricultural Science , Volume 120 , Issue 1 , February 1993 , pp. 107 - 114
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Agricultural Research Council (1984). Nutrient Requirements of Ruminant Livestock, Supplement No. 1. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Balcells, J., Guada, J. A., Castrillo, C. & Gasa, J. (1991). Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agricultural Science, Cambridge 116, 309–317.Google Scholar
Bauman, D. E., Davis, C. L. & Bucholtz, H. F. (1971). Propionate production in the rumen of cows fed either a control or high-grain, low-fiber diet. Journal of Dairy Science 54, 1282–1287.CrossRefGoogle ScholarPubMed
Chen, X. B., Hovell, F. D. DeB., Ørskov, E. R. & Brown, D. S. (1990). Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63, 131–142.CrossRefGoogle ScholarPubMed
Czerkawski, J. W. (1974). Methods for determining 2–6-diaminopimelic acid and 2-aminoethylphosphonic acid in gut contents. Journal of the Science of Food and Agriculture 25, 45–55.CrossRefGoogle ScholarPubMed
Coelho da Silva, J. F., Seeley, R. C., Beever, D. E., Prescott, J. H. D. & Armstrong, D. G. (1972). The effect in sheep of physical form and stage of growth on the sites of digestion of a dried grass. 2. Sites of nitrogen digestion. British Journal of Nutrition 28, 357–371.CrossRefGoogle Scholar
Faichney, G. J. (1975). The use of markers to partition digestion within the gastrointestinal tract of ruminants. In Digestion and Metabolism in Ruminants (Eds McDonald, I. W. & Warner, A. C. I.), pp. 277–291. Armidale: The University of New England Publication Unit.Google Scholar
Girdler, C. P., Thomas, P. C. & Chamberlain, D. G. (1986). Exogenous supply of glucose precursors and nitrogen utilization in sheep. Proceedings of the Nutrition Society 45, 43A.Google Scholar
Hannah, S. M., Stern, M. D. & Ehle, F. R. (1986). Evolution of a dual flow continuous culture system for estimating bacterial fermentation in vivo of mixed diets containing various soya bean products. Animal Feed Science and Technology 16, 51–62.Google Scholar
Koenig, S. E., Schelling, G. T., Mitchell, G. E. Jr, & Tucker, R. E. (1980). Purine and pyrimidine bases as potential indicators of microbial protein synthesis. Journal of Animal Science 51, Supplement No. 1. 25.Google Scholar
Ling, J. R. & Buttery, P. J. (1978). The simultaneous use of ribonucleic acid, 35S, 2,6-diaminopimelic acid and 2-aminoethylphosphonic acid as markers of microbial nitrogen entering the duodenum of sheep. British Journal of Nutrition 39, 165–179.Google Scholar
Lobley, G. E. (1991). Some interactions between protein and energy in ruminant metabolism. In Proceedings of the EAAP6th International Symposium on Protein Metabolism and Nutrition, Herning. Denmark (Eds Eggum, B. O., Boisen, S., Borsting, C., Danfær, A. & Hvelplund, T.), Vol. 1. pp. 66–79. Foulum: National Institute of Animal Science.Google Scholar
MacRae, J. C. & Lobley, G. E. (1986). Interactions between energy and protein. In Control of Digestion and Metabolism in Ruminants (Eds Milligan, L. P., Grovum, W. L. & Dobson, A.), pp. 367–385. Englewood Cliff, NJ: Prentice Hall.Google Scholar
MacRae, J. C. & Ulyatt, M. J. (1974). Quantitative digestion of fresh herbage by sheep. II. The sites of digestion of some nitrogenous constituents. Journal of Agricultural Science, Cambridge 82, 309–319.Google Scholar
MacRae, J. C., Smith, J. S., Dewey, P. J. S., Brewer, A. C., Brown, D. S. & Walker, A. (1985). The efficiency of utilization of metabolizable energy and apparent absorption of amino acids in sheep given spring- and autumn-harvested dried grass. British Journal of Nutrition 54, 197–209.CrossRefGoogle ScholarPubMed
McAllan, A. B. (1982). The fate of nucleic acids in ruminants. Proceedings of the Nutrition Society 41, 309–317.CrossRefGoogle ScholarPubMed
Nikolić, J. A. & Jovanović, M. (1973). Preliminary study on the use of different methods for determining the proportion of bacterial nitrogen in the total nitrogen of rumen contents. Journal of Agricultural Science, Cambridge 81, 1–7.CrossRefGoogle Scholar
Nolan, J. V. & Leng, R. A. (1972). Dynamic aspects of ammonia and urea metabolism in sheep. British Journal of Nutrition 27, 177–194.CrossRefGoogle ScholarPubMed
Reynolds, C. K. & Huntington, G. B. (1988). Partition of portal-drained visceral net flux in beef steers. 1. Blood flow and net flux of oxygen, glucose and nitrogenous compounds across stomach and post-stomach tissues. British Journal of Nutrition 60, 539–551.CrossRefGoogle ScholarPubMed
Seal, C. J. & Parker, D. S. (1991). Increased plasma free amino acid concentrations and net absorption of amino acids into portal and mesenteric veins with intraruminal propionate infusion into forage-fed steers. In Proceedings of the EAAP 6th International Symposium on Protein Metabolism and Nutrition, Herning, Denmark (Eds Eggum, B. O., Boisen, S., Børsting, C., Danfær, A. & Hvelplund, T.), Vol. 2. pp. 184–186. Foulum: National Institute of Animal Science.Google Scholar
Seal, C. J., Mole, J. L., Balcells, J. & Parker, D. S. (1991 a). Effect of intraruminal propionate infusion on nitrogen digestion in forage-fed sheep. In Proceedings of the EAAP 6th International Symposium on Protein Metabolism and Nutrition, Herning, Denmark (Eds Eggum, B. O., Boisen, S., Børsting, C., Danfær, A. & Hvelplund, T.), Vol. 2. pp. 187–189. Foulum: National Institute of Animal Science.Google Scholar
Seal, C. J., Roper, C. S. & Parker, D. S. (1991 b). Effect of rumen propionate infusion on rumen fermentation and whole body glucose turnover rate in forage-fed steers. Proceedings of the Nutrition Society 50, 78A.Google Scholar
Seal, C. J., Parker, D. S. & Avery, P. J. (1992). The effect of forage and forage-concentrate diets on rumen fermentation and metabolism of nutrients by the mesentericand portal-drained viscera in growing steers. British Journal of Nutrition 67, 355–370.CrossRefGoogle ScholarPubMed
Siddons, R. C., Nolan, J. V., Beever, D. E. & MacRae, J. C. (1985 a). Nitrogen digestion and metabolism in sheep consuming diets containing contrasting forms and levels of N. British Journal of Nutrition 54, 175–187.CrossRefGoogle ScholarPubMed
Siddons, R. C., Paradine, J., Beever, D. E. & Cornell, P. R. (1985 b). Ytterbium acetate as a particulate-phase digesta-flow marker. British Journal of Nutrition 54, 509–519.CrossRefGoogle ScholarPubMed
Smith, R. H. & McAllan, A. B. (1971). Nucleic acid metabolism in the ruminant. 3. Amounts of nucleic acids and total and ammonia nitrogen in digesta from the rumen, duodenum and ileum of calves. British Journal of Nutrition 25, 181–190.CrossRefGoogle ScholarPubMed
Smith, R. H., McAllan, A. B. & Hill, W. B. (1968). Nucleic acids in bovine nutrition. 2. Production of microbial amino acids in the rumen. Proceedings of the Nutrition Society 27, 48A.Google Scholar
Smith, R. H., Lewis, P. E. & McAllan, A. B. (1977). Estimation of microbial nitrogen compounds entering the ruminant duodenum using different reference components including a 32P label. Proceedings of the Nutrition Society 36, 6A.Google Scholar
Smith, R. H., McAllan, A. B., Hewitt, D. & Lewis, P. E. (1978). Estimation of amounts of microbial and dietary nitrogen compounds entering the duodenum of cattle. Journal of Agricultural Science, Cambridge 90, 557–568.CrossRefGoogle Scholar
Tagari, H. & Bergman, E. N. (1978). Intestinal disappearance and portal blood appearance of amino acids in sheep. Journal of Nutrition 108, 790–803.CrossRefGoogle ScholarPubMed
Tiemeyer, W. & Giesecke, D. (1982). Quantitative determination of allantoin in biological fluids by reversed-phase high-pressure liquid chromatography. Analytical Biochemistry 123, 11–13.CrossRefGoogle ScholarPubMed
Theurer, C. B. (1982). Microbial protein estimation using DAP, AEP and other amino acids as markers. In Protein Requirements for Cattle (Ed. Owens, F. N.), pp. 10–22. Oklahoma: Oklahoma State University.Google Scholar
Veenhuizen, J. J., Russell, R. W. & Young, J. W. (1988). Kinetics of metabolism of glucose, propionate and CO2 steers as affected by injecting phlorizin and feeding propionate. Journal of Nutrition 118, 1366–1375.CrossRefGoogle ScholarPubMed
Webster, A. J. F. (1980). Energy costs of digestion and metabolism in the gut. In Digestive Physiology and Metabolism in Ruminants (Eds Ruckebusch, Y. & Thivend, P.), pp. 469–484. Lancaster: MTP Press.Google Scholar
Williams, C. H., David, D. J. & Iismaa, O. (1962). The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. Journal of Agricultural Science, Cambridge 59, 381–385.Google Scholar
Work, E. & Dewey, D. L. (1953). The distribution of ɛ-diaminopimelic acid among various micro-organisms. Journal of General Microbiology 9, 394–409.CrossRefGoogle Scholar