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Introgression of isozyme genes utilizing monosomic alien addition lines (MAALs) of Oryza officinalis in O. sativa

Published online by Cambridge University Press:  16 July 2001

P. KAUSHAL
Affiliation:
Department of Genetics, Punjab Agricultural University, Ludhiana 141 004, Punjab, India Present address: C.I. Division, Indian Grassland and Fodder Research Institute, Jhansi 284 003, U.P., India. Email: pkaushal@mailcity.com
RAVI
Affiliation:
Department of Genetics, Punjab Agricultural University, Ludhiana 141 004, Punjab, India
J. S. SIDHU
Affiliation:
Department of Genetics, Punjab Agricultural University, Ludhiana 141 004, Punjab, India
H. S. DHALIWAL
Affiliation:
Department of Genetics, Punjab Agricultural University, Ludhiana 141 004, Punjab, India

Abstract

Starch gel electrophoresis was carried out on ten monosomic alien addition lines (MAALs) of Oryza officinalis (CC genome) in O. sativa (AA genome) at Punjab Agricultural University, Ludhiana, India during 1993–1995. The diploid progeny from MAALs were studied for possible gene transfer of isozyme genes. Analysis showed introgression for alleles of wild species for peroxidase, esterase and acid phosphatase isozymes in progeny of MAAL 2, esterase in MAAL 7 and acid phosphatase in MAAL 6, indicating partial homology between the two genomes. MAAL 2 yielded the highest number of recombinants. The potential of addition lines for alien gene transfer as well as for linkage mapping is discussed.

Type
Research Article
Copyright
© 2001 Cambridge University Press

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Introgression of isozyme genes utilizing monosomic alien addition lines (MAALs) of Oryza officinalis in O. sativa
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