Hostname: page-component-848d4c4894-75dct Total loading time: 0 Render date: 2024-05-12T07:42:15.316Z Has data issue: false hasContentIssue false

Time-mortality response in Spodoptera exempta (Walk.) infected with heat-treated nuclear polyhedrosis virus

Published online by Cambridge University Press:  19 September 2011

M. O. Odindo
Affiliation:
Department of Zoology, University of Nairobi, P.O. Box 30197, Nairobi, Kenya*
Get access

Abstract

The susceptibility of third-instar Spodoptera exempta (Walk.) to a heat-treated nuclear polyhedrosis virus was tested in the laboratory. The polyhedral suspension was purified by differential centrifugation and heat-treated at temperatures that ranged from 20 to 90°C for 10–30 min. The LT50 ranged from 108.4 hr for virus heated at 50°C (10 min) to 162.2 hr for virus heated at 80°C (10 min). The fiducial limits to the LD50s were calculated at 0.95 probability.

Type
Research Article
Copyright
Copyright © ICIPE 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Brown, E. S. (1970) Control of the African armyworm Spodoptera exempta (Walk.)—an appreciation of the problem. E. Afr. agric.for. J. 35, 237245.Google Scholar
Brown, E. S. and Swaine, G. (1965) Virus disease of the African armyworm Spodoptera exempta (Walk.). Bull. ent. Res. 56, 95116.Google Scholar
Finney, D. J. (1971) Probit Analysis. Cambridge University Press, London.Google Scholar
Gudauskas, R. T. and Canerday, D. (1968) The effect of heat, buffer, salt and H-ion concentration, and ultraviolet light on the infectivity of Heliothis and Trichoplusia nuclear polyhedrosis viruses. J. Invertebr. Pathol. 12, 405411.Google Scholar
Lathika, P. and Jacob, A. (1974) Effect of temperature and sunlight on the infectivity of a nuclear polyhedrosis virus of Spodoptera mauritia (Boisduval). Curr. Sei. 43, 587588.Google Scholar
Macfarlane, J. J. Jr, and Keeley, L. (1969) Heat effect on the infectivity of the nuclear polyhedrosis virus of Heliothis species. J. econ. Entomol. 62, 925929.Google Scholar
Martignoni, M. E. and Iwai, P. J. (1977) Thermal inactivation characteristics of two strains of nucleopolyhedrosis virus (Baculovirus subgroup A) pathogenic for Orgia pseudotsugata. J. Invertebr. Pathol. 30, 255262.Google Scholar
Nair, K. P. V. and Jacob, A. (1976) Studies on the nuclear polyhedrosis of Pericallia ricini F. (Lep: Arctidae). Entomon. 1, 2330.Google Scholar
Pawar, V. M. and Ramakrishnan, N. (1971) Investigations on the nuclear-polyhedrosis of Prodenia litura F. II. Effect of surface disinfectants temperature and alkali on the virus. Indian J. Entomol. 33, 428432.Google Scholar
Stuermer, J. R. C. W. and Bullock, H. R. (1968) Thermal inactivation of Heliothis nuclear polyhedrosis virus. J. Invertebr. Pathol. 12, 473474.Google Scholar
Tanada, Y. (1967) Effect of high temperatures on the resistance of insects to infectious diseases. J. Sericult. Sei. Japan. 36, 333339.Google Scholar