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Storability of five new formulations of Green Muscle® (Metarhizium acridum) under ambient and low temperatures: evaluation of conidial viability and virulence against desert locust nymphs

Published online by Cambridge University Press:  11 June 2013

Mohamed Ould Etheimine*
Affiliation:
Centre National de Lutte Antiacridienne, BP 665, Nouakchott, Mauritanie
Cherif Mohamed Habib Kane
Affiliation:
Centre National de Lutte Antiacridienne, BP 665, Nouakchott, Mauritanie
Sidi Ould Ely
Affiliation:
Centre National de Lutte Antiacridienne, BP 665, Nouakchott, Mauritanie
Adama Barry
Affiliation:
Centre National de Lutte Antiacridienne, BP 665, Nouakchott, Mauritanie
Sid'ahmed Ould Mohamed
Affiliation:
Centre National de Lutte Antiacridienne, BP 665, Nouakchott, Mauritanie
Mohamed Abdallahi Ould Babah
Affiliation:
Centre National de Lutte Antiacridienne, BP 665, Nouakchott, Mauritanie
Mounsif Benchekroun
Affiliation:
Laboratoire d'Agro-alimentaire et Santé, Université Hassan 1er, Faculté des Sciences et Techniques, BP 577, 2600Settat, Maroc
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Abstract

The storability of five new different formulations of Metarhizium acridum (two oil, one paste, one semi-solid and one powder) was investigated under ambient and low temperatures. Fourth-instar nymphs of the desert locust Schistocerca gregaria (Forskål) were used to evaluate their virulence. Under ambient conditions (23–38°C), all the formulations maintained acceptable viability (66.1 ± 2.30 to 71.8 ± 0.88% germination) after 6 months of storage. After 10 months, only conidia from the powder formulation remained viable. In contrast, samples of the formulations kept under low temperatures ( − 1.2 to 15°C) did not show significant loss of viability after 30 months of storage despite repeated power cuts, lasting sometimes several days. During this period, M. acridum conidia remained virulent to nymphs with 100% mortality 5 to 7 days after the treatment. These results show that all the formulations have greater stability when stored under low and even fluctuant temperature changes. Under ambient conditions, the formulations could remain effective for at least 6 months of storage.

Type
Research Papers
Copyright
Copyright © icipe 2013 

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