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The isolation and identification of the cuticular sex-stimulant pheromone of the tsetse Glossina pallidipes Austen (Diptera:Glossinidae)

Published online by Cambridge University Press:  19 September 2011

P. G. McDowell ,
D. L. Whitehead ,
M. F. B. Chaudhury  and
W. F. Snow
P. G. McDowell
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
D. L. Whitehead
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
M. F. B. Chaudhury
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
W. F. Snow
Affiliation:
ICIPE Muhaka Field Station, P.O. Box 80804, Mombasa, Kenya
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Abstract

The sex-stimulant contact pheromone present in the cuticle of female tsetse Glossina pallidipes Austen has been extracted with hexane, separated by preparative gas chromatography and identified by mass spectrometry. The aphrodisiac, consisting of 13,17-dimethylpentatriacontane and 15,19-dimethylpentatriacontane, is present in cuticular extracts of 1-week-old females and, in lower amounts, in teneral females but it is almost absent in males of all ages. Full copulatory responses to hexane-washed dead males treated with the two dimethylpentatriacontane isomers were induced in 10–20-day-old adult male G. pallidipes. The practical uses to which this discovery can now be put—towards the mass-rearing and control of G. pallidipes— are discussed in detail.

Keywords

TsetseGlossina pallidipespheromonesex-stimulantdimethylpentatriacontanesauto-chemosterilization
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 2 , Issue 3 , September 1981 , pp. 181 - 187
Copyright
Copyright © ICIPE 1981

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References

REFERENCES

Baker, R., Bradshaw, J. W. S., Evans, D. A., Higgs, M. D. and Wadhams, J. (1976) An efficient all-glass splitter and trapping system for gas chromatography. J. Chromat. Sci. 14, 425–27.CrossRefGoogle Scholar
Blomquist, G. J., Blailock, T. T., Scheetz, R. W. and Jackson, L. L. (1976) Cuticular lipids of insects—VII. Cuticular hydrocarbons of the crickets Acheta domesticus, Gryllus Pennsylvania and Nemobius fasciatus. Comp. Biochem. Physiol. 54B, 381–386.Google Scholar
Carlson, D. A., Langley, P. A. and Huyton, P. (1978) Sex pheromone of the tsetse fly: isolation, identification, and synthesis of contact aphrodisiacs. Science 201, 750–753.CrossRefGoogle ScholarPubMed
Challier, A. (1965) Amélioration de la méthode de détermination de l'âge physiologique des glossines. Etudes faites sur Glossina palpalis gambiensis Vanderplank, 1949. Bull. Soc. Path. exot. 58, 250–259.Google Scholar
Challier, A. (1973) Ecologic de Glossina palpalis gambiensis Vanderplank, 1949 (Diptera:Muscidae) en savane d'Afrique occidentale. Máem. ORSTOM, Paris 64, 274.Google Scholar
Challier, A. (1977) Trapping technology. In Tsetse, The Future for Biological Methods in Integrated Control (Ed. by Laird, M.), IDRC-077e, Ottawa.Google Scholar
Challier, A. and Gouteux, J.-P. (1978) Enquêtes entomologiques dan le foyer de maladic du Sommeil de Vavous—Republiques de Côte d'lvoire (jan-mar 1978). II. Possibilitiés et essais de lutte en zone forestière contreGlossina palpalis palpalis (R-D). Rapport OCCGECentre Muraz, number 20/ENT. 78.Google Scholar
Challier, A. and Laveissiere, C. (1973) Un nouveau piège pour la capture des glossines (Glossina:Diptera. Muscidae): description et essais sur le terrain. Cah. O.R.S.T.O.M. Sér. Ent. med. Parasit. 11, 252–262.Google Scholar
Harley, J. M. N. (1966) Studies on age and trypanosome infection rate in females of Glossina pallidires Aust., G. palpalis fuscipes Newst, and G. brevipalpis Newst, in Uganda. Bull. ent. Res. 57, 23–37.CrossRefGoogle Scholar
Howard, R. W., McDaniel, C. A., Nelson, D. R. and Blomquist, G. J. (1980) Chemical ionisation mass spectrometry. Application to insect derived cuticular alkanes. J. chem. Ecol. 6, 609–623.CrossRefGoogle Scholar
Huyton, P. M., Langley, P. A., Carlson, D. A. and Coates, T. W. (1980a) The role of sex pheromones in initiation of copulatory behaviour by male tsetse flies, Glossina morsitans morsitans. Physiol. Ent. 5, 243–252.CrossRefGoogle Scholar
Huyton, P. M., Langley, P. A., Carlson, D. A. and Schwarz, M. (1980b) Specificity of contact sex pheromones in tsetse flies Glossina spp. Physiol. Ent. 5, 253–264.CrossRefGoogle Scholar
Jaenson, T. G. T. (1980) Mating behaviour of females of Glossina pallidipes Austen (Diptera: Glossinidae). Bull, ent. Res. 70, 49–60.Google Scholar
Jordan, A. M. (1958) The mating behaviour of females of Glossina palpalis (R-D) in captivity. Bull. ent. Res. 49, 35–43.CrossRefGoogle Scholar
Kovats, E. (1965) Gas chromatographic characterization of organic substances and the retention index system. In Advances in Chromatography (Ed. by Giddings, J. C.), Vol. 1, Chap. 7, pp. 229–247.Google Scholar
Langley, P. A. (1977) Physiology of tsetse flies (Glossina spp.) (Diptera:Glossinidae): a review. Bull. ent. Res. 67, 527–574.CrossRefGoogle Scholar
Langley, P. A., Carlson, D. A.Huyton, P. M. and Coates, T. W. (1979) Prospects for use of specific sex pheromones for tsetse control. 16th Meeting of International Scientific Council for Trypanosomiasis Research and Control, Yaounde, Cameroun.Google Scholar
Langley, P. A., Pimley, R. W. and Carlson, D. A. (1975) Sex recognition pheromone in tsetse fly Glossina morsitans. Nature, Lond. 254, 51–53.CrossRefGoogle ScholarPubMed
Nash, T. A. M., Jordan, A. M. and Trewern, M. A. (1971) Mass rearing of tsetse flies (Glossina spp.): present advances. In Sterility Principle for Insect Control or Eradication (publication No. sII/PUB/265), pp. 99–110. International Atomic Energy Agency, Vienna.Google Scholar
Okiwelu, S. N. (1977) Insemination, pregnancy and suspected abortion rates in a natural population of Glossina morsitans morsitans (Diptera:Glossinidae) in the Republic of Zambia. J. med. Ent. 14, 19–23.CrossRefGoogle Scholar
Rogers, A. (1972) Studies on the mating of Glossina pallidipes Austen. I. The age at mating. Ann. trap. Med. Parasit. 66, 515–523.CrossRefGoogle Scholar
Saunders, D. S. (1960) Ovulation cycle in Glossina morsitans Westwood (Diptera:Muscidae) and a possible method of age determination for female tsetse flies by the examination of their ovaries. Trans. R. ent. Soc. Lond. 112, 221–238.CrossRefGoogle Scholar
Saunders, D. S. (1962) Age determination for female tsetse flies and the age compositions of samples of Glossina pallidipes (Aust., G. palpalis fuscipes Newst and G. brevipalpis Newst. Bull. ent. Res. 53, 579–595.CrossRefGoogle Scholar
Vale, G. A. and Hargrove, J. W. (1975) Field attraction of tsetse flies (Diptera: Glossinidae) to ox odour; the effect of dose. Proc. Trans. Rhod. scient. Ass. 56, 46–50.Google Scholar
van Etten, J. (1981) A comparison of the performance of laboratory colonies of Glossina pallidipes Austen from two allotropic populations in Kenya. Insect Sci. Applic. 1, 177–183.Google Scholar
Whitehead, D. L. (1981) The effect of phytosterols on tsetse reproduction. Insect Sci. Applic. 1, 281–288.Google Scholar