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Identification of Behaviourally Active Components from Maize Volatiles for the Stemborer Parasitoid Cotesia flavipes Cameron (Hymenoptera: Braconidae)

Published online by Cambridge University Press:  19 September 2011

Adele J. Ngi-Song ,
Peter G. N. Njagi ,
Baldwyn Torto  and
William A. Overholt
Adele J. Ngi-Song
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Peter G. N. Njagi
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
Baldwyn Torto
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
William A. Overholt
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya
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Abstract

In the present study, Y-tube olfactometric assays confirmed that volatiles from maize seedlings infested with Chilo partellus larvae were more attractive to the borer's larval endoparasitoid Cotesia flavipes, than volatiles from uninfested maize. Coupled gas chromatography-electroantennographic detector (GC-EAD) analysis of the volatiles from larvae-infested maize revealed six electrophysiologically active compounds on the antennae of the female parasitoid. These compounds were identified by GC-MS as (Z)-3-hexenyl acetate, linalool, (E)-4, 8-dimethyl-1, 3, 7-nonatriene, heptanal, (E)-β-ocimene and a C-5 aliphatic compound. (E)-4, 8-Dimethyl-1, 3, 7-nonatriene was present in EAG-detectable amounts in the volatiles of uninfested seedlings. In bioassays, a blend comprised of (Z)-3-hexenyl acetate, linalool, (E)-4, 8-dimethyl-1,3,7-nonatriene, (E)-β-ocimene and heptanal was significantly attractive to the parasitoid. Of the individual compounds, (Z)-3-hexenyl acetate was attractive at the doses tested while (E)-4,8-dimethyl-1,3,7-nonatriene and heptanal showed varying degree of attractiveness to the parasitoid at different doses. Linalool and (E)-β-ocimene were unattractive at the same doses. The significance of these results is discussed.

Résumé

Dans cette étude, des essais olfactométriques en tubes Y ont confirmé que les matières volatiles de jeunes plants de maïs infestés par des larves du foreur de tige, Chilo partellus attiraient plus de larves de Cotesia flavipes un endoparasitoïde de ce foreur, en comparaison des matières volatiles du maïs non infesté. L'analyse par chromatographie à phase gazeuse couplée avec un détecteur électro-antennographique des matières volatiles du maïs infesté de larves a révélé six composés avec des activités éléctro-physiologiques sur les antennes de la femelle du parasitoïde. Ces composés ont été identifiés par méthodes GC-MS comme (Z)-3-hexényle acétate, linalol, (E)-4, 8-diméthyle-1,3,7-nonatriène, heptanal, (E)-β-ocimène et un autre composé, le C-5 aliphatique. (E)-4, 8-diméthyle-1,3,7-nonatriène était présent en quantités détectables par méthode EAG, dans les matières volatiles de jeunes plants non infestés. Au cours des essais biologiques, un cocktail fait de (Z)-3-hexényle acétate, de linalool, de (E)-4,8-diméthyle-1,3,7-nonatriène, de (E)-β-ocimène et d'heptanal, attirait fortement le parasitoïde. Quant aux différents composés pris isolément, (Z)-3-hexényle acétate était attrayant aux doses testées tandis que (E)-4, 8-diméthyle-l, 3, 7-nonatriène et heptanal montraient des degrés variables d'attractivité à différentes doses. Le linalol et (E)-β-ocimène ne montraient pas d'attrait aux mêmes doses. L'article discute de la signification des résultats obtenus.

Keywords

Chilo partellustritrophic systemCotesia flavipesGC-EADZen maysplant volatilesBraconidaePyralidaeChilo partellussystème tritrophiqueCotesia flavipesGC-EADZea maismatières volatiles végétalesBraconidaePyralidae
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 20 , Issue 3 , September 2000 , pp. 181 - 189
Copyright
Copyright © ICIPE 2000

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