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Experimental transmission of Ife virus by Aedes aegypti mosquitoes

Published online by Cambridge University Press:  19 September 2011

G. O. Ezeifeka ,
J. U. Umoh ,
C. D. Ezeokoli  and
N. E. Gomwalk
G. O. Ezeifeka
Affiliation:
Department of Veterinary Public Health and Preventive Medicine, Zaria, Nigeria
J. U. Umoh
Affiliation:
Department of Veterinary Public Health and Preventive Medicine, Zaria, Nigeria
C. D. Ezeokoli
Affiliation:
Department of Veterinary Surgery and Medicine, Zaria, Nigeria
N. E. Gomwalk
Affiliation:
Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria
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Abstract

Transmission of Ife virus by Aedes aegypti mosquitoes was investigated. Mosquitoes were experimentally infected by feeding on viraemic mice or on infected mouse brain suspended in defibrinated chick blood. Normal infant mice were exposed to infected mosquitoes after predetermined extrinsic incubation period. Ife virus persisted for only 1 day in mosquitoes that fed on viraemic mice and for 5 days in those that fed on infected brain/blood suspension. Virus was not recovered in infant mice exposed to infected mosquitoes. However, there was serological evidence of virus transmission manifested as the presence of complement fixing antibody against Ife virus in mice exposed after 2 days and 2–5 days extrinsic incubation periods to mosquitoes infected by feeding on viraemic mice and by feeding on infected brain/blood suspension respectively. This transmission was considered mechanical in view of the short virus persistence and extrinsic incubation periods.

Résumé

La transmission du virus d'Ife par les moustlques de l'espèce Aedes aegypti a été étudiée. Ces moustiques ont etc experimentalement infectés en les nourrisant de souris viraemiques, ou de matlère cérébrale (cervelle) de souris infectées dans une suspension de sang defibriné de poulet. Des jeunes souris ont été exposées aux moustiques infectés apres la pcriode d'incubation extrinsique prédéterminée.

Dans les moustiques nourris de souris viraemiques, le virus d'Ife n'a persistc qu 'un Jour, contre cinq jours pour ceux nourris de la suspension infectee cervelle/sang. Aucun virus n'a été retrouvé dans les jeunes souris exposées aux moustiques infectés. Cependant, on a pu trouver une evidence de transmission du virus, ceci se manifestant par la presence d'anticorps complémentaires contre le virus d'Ife dans les souris exposées durant des périodes d'incubation extrinsèques de 2 jours et de 2 à 5 jours aux moustiques infectés en les nourrisant de souris viraemiques et de la suspension infectee cervelle/sang respectivement. Cette transmission est consklérée comme étant mécanique à cause de les courte durée de la persistence et des périodes d'incubation extrinsèques.

Keywords

Aedes aegyptiIfe virustransmission
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 14 , Issue 1 , February 1993 , pp. 89 - 92
Copyright
Copyright © ICIPE 1993

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