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Molecular Heterogeneity of Acinetobacter baumanii Isolates During Seasonal Increase in Prevalence

Published online by Cambridge University Press:  02 January 2015

Celia Christie ,
Dorothy Mazon ,
Walter Hierholzer Jr.  and
Jan Evans Patterson
Celia Christie
Affiliation:
Divisions of Infectious Diseases and, Hospital Epidemiology, New Haven, Connecticut
Dorothy Mazon
Affiliation:
Children's Hospital Medical Center, Cincinnati, Ohio, and the Department of Medicine (Infectious Diseases), New Haven, Connecticut
Walter Hierholzer Jr.
Affiliation:
Children's Hospital Medical Center, Cincinnati, Ohio, and the Department of Medicine (Infectious Diseases), New Haven, Connecticut
Jan Evans Patterson
Affiliation:
Children's Hospital Medical Center, Cincinnati, Ohio, and the Department of Medicine (Infectious Diseases), New Haven, Connecticut
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Abstract

Objective:

To study the epidemiology of Acinetobacter baumanii during a period of increased seasonal prevalence.

Design:

Descriptive molecular and clinical epidemiologic study of A baumanii isolates from 1990 through 1992.

Setting:

A 770-bed urban, acute, tertiary-care university hospital.

Results:

During 1990 through 1992, the rate of A baumanii isolations was 30.4 per 1,000 culture isolations during the summer, compared to 12.6 per 1,000 culture isolations during the fall, winter, and spring (P<.000001). There were 320 isolates identified among 260 patients during this time. Eighty-one patients with isolates available were identified for evaluation; they ranged in age from 2 months to 95 years. Pulsed-field gel electrophoresis (PFGE) typing showed that 59 (83%) of 71 typed isolates had distinct PFGE patterns. There were three small clusters of isolates with the same PFGE patterns, suggesting cross-transmission in those instances.

Conclusions:

A seasonally increased prevalence of A baumanii largely associated with device-related nosocomial infections in severely ill patients was noted over a 3-year period. Although there were isolated instances of cross-transmission, most isolates had distinct PFGE patterns. Clonal dissemination of a single strain was not responsible for the seasonal increased prevalence of A baumanii. PFGE typing was useful in directing control efforts.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 16 , Issue 10 , October 1995 , pp. 590 - 594
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1995

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References

1. Centers for Disease Control and Prevention. National Nosocomial Infections Surveillance System. Semiannual report. Summary of NNIS data. May 1993, p 5.Google Scholar
2. Smith, PW, Massanari, RM. Room humidifiers as the source of acinetobacter infections. JAMA 1977;237:795–797.CrossRefGoogle ScholarPubMed
3. Sheretz, RJ, Sullivan, M. An outbreak of infections with Acinetobacter baumanii in burn patients: contamination of patients' mattresses. J Infect Dis 1985;151:252–258.CrossRefGoogle Scholar
4. Abrutyn, E, Goodhart, GL, Roos, K, Anderson, R, Buxton, A. Acinetobacter calcoaceticus outbreak associated with peritoneal dialysis. Am J Epidemiol 1978;107:328–335.Google Scholar
5. Patterson, JE, Vecchio, J, Pantelick, E, et al. Association of contaminated gloves with transmission of Acinetobacter calcoaceticus var. anitratus in an intensive care unit. Am J Med 1991;91:479–483.Google Scholar
6. Bouvet, PJM, Jeanjean, S, Vieu, JF, Dijkshoorn, L. Species, bio-type and bacteriophage type determinations compared with cell envelope protein profiles for typing Acinetobacter strains. J Clin Microbiol 1990;28:170–176.Google Scholar
7. Towner, KJ, Chopade, BA. Biotyping of Acinetobacter calcoaceticus using API zone system. J Hosp Infect 1987;10:145–151.CrossRefGoogle Scholar
8. Traub, WH. Acinetobacter baumannii serotyping for delineation of outbreaks of nosocomial cross-infection. J Clin Microbiol 1989;27:2713–2716.Google Scholar
9. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. M7-T2. Villanova, PA: National Committee for Clinical Laboratory Standards, 1988.Google Scholar
10. Garner, JS, Jarvis, WR, Emori, TG, Horan, TC, Hughes, JM. CDC definitions for nosocomial infections, 1988. Am J Infect Control 1988;16:128–140.Google Scholar
11. Smith, CL, Cantor, CR. Purification, specific fragmentation and separation of large DNA molecules. Methods Enzymology 1987;155:449–467.Google Scholar
12. Murray, BE, Singh, KV, Heath, JD, Sharma, BR, Weinstock, GM. Comparison of genomic DNAs of different enterococcal isolates using restriction endonuclease with infrequent recognition sites. J Clin Microbiol 1990;28:2059–2063.CrossRefGoogle ScholarPubMed
13. Centers for Disease Control. Acinetobacter calcoaceticus: a nosocomial pathogen with an unusual seasonal pattern. J Infect Dis 1979;139:371–375.CrossRefGoogle Scholar
14. Ramphal, R, Kluge, RM. Acinetobacter calcoaceticus: an increasing nosocomial problem. Am J Med Sci 1979;277:57–66.CrossRefGoogle ScholarPubMed
15. Edberg, SC, Patterson, JE, Smith, DB. Differentiation of distribution systems, source water, and clinical coliforms by DNA analysis. J Clin Microbiol 1994;32:139–142.Google Scholar
16. Glew, RH, Moellering, RC Jr Kunz, LJ. Infections with Acinetobacter calcoaceticus clinical and laboratory studies. Medicine 1977;56:79–97.Google Scholar
17. Murray, BE, Moellering, RC Jr Aminoglycoside-modifying enzymes among clinical isolates of Acinetobacter baumanii: explanation for high-level aminoglycoside antibiotic resistance. Antimicrob Agents Chemother 1979;15:190–199.Google Scholar
18. Devaud, M, Kayser, FG, Bachi, B. Transposon-mediated multiple antibiotic resistance in Acinetobacter strains. Antimicrob Agents Chemother 1982;22:323–329.CrossRefGoogle ScholarPubMed
19. Gouby, A, Carles-Nurit, MJ, Bouziges, N, Bourg, G, Mesnard, R, Bouvet, PJ. Use of pulsed-field gel electrophoresis for investigation of hospital out breaks of Acinetobacter baumanii . J Clin Microbiol 1992;30(6):1588–1591.CrossRefGoogle Scholar
20. Kropec, A, Hubner, J, Daschner, FD. Comparison of three typing methods in hospital outbreaks of Acinetobacter calcoaceticus . J Hosp Infect 1993;23(2):133–141.Google Scholar
21. Struelens, MJ, Carlier, E, Maes, N, Serruys, E, Quint, WJ, van Belkum, A. Nosocomial colonization and infection with multiresistant Acinetobacter baumanii: outbreak delineation using macrorestriction analysis and PCR-fingerprinting. J Hosp Infect 1993;25(1):15–32.CrossRefGoogle Scholar