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Efficient Recovery of Fluoroquinolone-Susceptible and Fluoroquinolone-Resistant Escherichia coli Strains From Frozen Samples

Published online by Cambridge University Press:  02 January 2015

Ebbing Lautenbach ,
Evelyn Santana ,
Abby Lee ,
Pam Tolomeo ,
Nicole Black ,
Andrew Babson ,
Eli N. Perencevich ,
Anthony D. Harris ,
Catherine A. Smith  and
Joel Maslow
Ebbing Lautenbach*
Affiliation:
Division of Infectious Diseases of the Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania Department of Biostatistics and Epidemiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania Center for Education and Research on Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania
Evelyn Santana
Affiliation:
Section of Infectious Diseases, Department of Veterans Affairs, Philadelphia, Pennsylvania
Abby Lee
Affiliation:
Section of Infectious Diseases, Department of Veterans Affairs, Philadelphia, Pennsylvania
Pam Tolomeo
Affiliation:
Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania
Nicole Black
Affiliation:
Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania
Andrew Babson
Affiliation:
Section of Infectious Diseases, Department of Veterans Affairs, Philadelphia, Pennsylvania
Eli N. Perencevich
Affiliation:
Veterans Affairs Maryland Health Care System, Baltimore Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore
Anthony D. Harris
Affiliation:
Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore
Catherine A. Smith
Affiliation:
Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania
Joel Maslow
Affiliation:
Division of Infectious Diseases of the Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania Section of Infectious Diseases, Department of Veterans Affairs, Philadelphia, Pennsylvania
*
University of Pennsylvania School of Medicine, Center for Clinical Epidemiology and Biostatistics, 825 Blockley Hall, 423 Guardian Drive, Philadelphia, PA 19104-6021 (ebbing@mail.med.upenn.edu)
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Abstract

We assessed the rate of recovery of fluoroquinolone-resistant and fluoroquinolone-susceptible Escherichia coli isolates from culture of frozen perirectal swab samples compared with the results for culture of the same specimen before freezing. Recovery rates for these 2 classes of E. coli were 91% and 83%, respectively. The majority of distinct strains recovered from the initial sample were also recovered from the frozen sample. The strains that were not recovered were typically present only in low numbers in the initial sample. These findings emphasize the utility of frozen surveillance samples.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 29 , Issue 4 , April 2008 , pp. 367 - 369
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2008

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