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Determining the Significance of Coagulase-Negative Staphylococci Identified in Cultures of Paired Blood Specimens from Neonates by Species Identification and Strain Clonality

Published online by Cambridge University Press:  21 June 2016

Yhu-Chering Huang ,
Ying-Hsiang Wang ,
Lin-Hui Su ,
Yi-Hong Chou ,
Rey-In Lien  and
Tzou-Yien Lin
Yhu-Chering Huang*
Affiliation:
Division of Pediatric Infectious Diseases, Chang Gung Children's Hospital, Kweishan, Taoyuan, Taiwan
Ying-Hsiang Wang
Affiliation:
Division of Neonatology, Chang Gung Children's Hospital, Kweishan, Taoyuan, Taiwan
Lin-Hui Su
Affiliation:
Department of Clinical Pathology, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan
Yi-Hong Chou
Affiliation:
Division of Neonatology, Chang Gung Children's Hospital, Kweishan, Taoyuan, Taiwan
Rey-In Lien
Affiliation:
Division of Neonatology, Chang Gung Children's Hospital, Kweishan, Taoyuan, Taiwan
Tzou-Yien Lin
Affiliation:
Division of Pediatric Infectious Diseases, Chang Gung Children's Hospital, Kweishan, Taoyuan, Taiwan
*
Chang Gung Children's Hospital, 5, Fu-Shin St, Kweishan, Taoyuan, Taiwan (ychuang@adm.cgmh.org.tw)
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Abstract

Of 13 paired coagulase-negative staphylococci (CoNS) isolates recovered from cultures of paired blood specimens obtained simultaneously from peripheral sites from neonates with clinical sepsis, 12 were identical species with identical genotypes. Isolation of CoNS in cultures of 2 blood samples obtained simultaneously from separate sites in neonates usually represents true infection.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 1 , January 2006 , pp. 70 - 73
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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References

1.Beck-Sague, CM, Azimi, P, Fonseca, SN, et al. Bloodstream infections in neonatal intensive care unit patients: results of a multicenter study. Pediatr Infect Dis J 1994; 13:1110–1116.CrossRefGoogle ScholarPubMed
2.Stoll, BJ, Gordon, T, Korones, SB, et al. Late-onset sepsis in very low birthweight neonates: a report from the National Institute of Child Health and Human Development Neonatal Research Network. J Pediatr 1996; 129:63–71.CrossRefGoogle ScholarPubMed
3.Gaynes, RP, Edwards, JR, Jarvis, WR, et al. Nosocomial infections among neonates in high-risk nurseries in the United States. Pediatrics 1996; 98: 357–361.CrossRefGoogle ScholarPubMed
4.Kirchhoff, LV, Sheagren, JN. Epidemiology and clinical significance of blood cultures positive for coagulase-negative staphylococcus. Infect Control 1985; 6:479–486.CrossRefGoogle ScholarPubMed
5.Baltimore, RS. Is it real or is it a contaminant? A guide to the interpretation of blood culture results. Am J Dis Child 1987; 141:241–242.Google ScholarPubMed
6.Archer, GL. Coagulase-negative staphylococci in blood cultures: the clinician's dilemma. Infect Control 1985; 6:477–478.CrossRefGoogle ScholarPubMed
7.Bates, DW, Lee, TH. Rapid classification of positive blood cultures: prospective validation of a multivariate algorithm. JAMA 1992; 267:1962–1966.CrossRefGoogle ScholarPubMed
8.Herwaldt, LA, Geiss, M, Kao, C, Pfaller, MA. The positive predictive value of isolating coagulase-negative staphylococci from blood cultures. Clin Infect Dis 1996; 22:14–20.CrossRefGoogle ScholarPubMed
9.St. Geme, JW III, Bell, LM, Baumgart, S, D'Angio, CT, Harris, MC. Distinguishing sepsis from blood culture contamination in young infants with blood cultures growing coagulase-negative staphylococci. Pediatrics 1990; 86:157–162.CrossRefGoogle ScholarPubMed
10.Archer, GL, Karchmer, AW, Vishniavsky, N, Johnston, JL. Plasmid-pattern analysis for the differentiation of infecting from noninfecting Staphylococcus epidermidis. J Infect Dis 1984; 149:913–920.CrossRefGoogle ScholarPubMed
11.Tan, TQ, Musser, JM, Shulman, Rj, Mason, EOJ, Mahoney, DHJ, Kaplan, SL. Molecular epidemiology of coagulase-negative Staphylococcus blood isolates from neonates with persistent bacteremia and children with central venous catheter infections. J Infect Dis 1994; 169:1393–1397.CrossRefGoogle ScholarPubMed
12.van Belkum, A, Kluijtmans, J, van Leeuwn, W, Goessens, W, ter Averst, E, Verbrugh, H. Investigation into the repeated recovery of coagulase-negative staphylococci from blood taken at the end of cardiopulmonary bypass. J Hosp Infect 1995; 31:285–293.CrossRefGoogle Scholar
13.Dominguez, MA, Linares, J, Pulido, A, Perez, JL, de Lencastre, H. Molecular tracking of coagulase-negative staphylococcal isolates from catheter-related infections. Microb Drug Resist 1996; 2:423–429.CrossRefGoogle ScholarPubMed
14.Khatib, P, Riederer, KM, Clark, JA, Khatib, S, Briski, LE, Wilson, FM. Coagulase-negative staphylococci in multiple blood cultures: strain relatedness and determinants of same-strain bacteremia. J Clin Microbiol 1995; 33:816–820.CrossRefGoogle ScholarPubMed
15.Zaidi, AE, Harrell, Q, Rost, JR, Reller, LB. Assessment of similarity among coagulase-negative staphylococci from sequential blood cultures of neonates and children by pulsed-field gel electrophoresis. J Infect Dis 1996; 174:1010–1014.CrossRefGoogle ScholarPubMed
16.Kim, S, McDonald, LC, Jarvis, WR, et al. Determining the significance of coagulase-negative staphylococci isolated from blood cultures at a community hospital: a role for species and strain identification. Infect Control Hosp Epidemiol 2000; 21:213–217.CrossRefGoogle Scholar
17.Huang, YC, Su, LH, Wu, TL, et al. Outbreak of Acinetobacter baumannii bacteremia in a neonatal intensive care unit: clinical implications and genotyping analysis. Pediatr Infect Dis J 2002; 21:1105–1109.CrossRefGoogle Scholar
18.Su, LH, Leu, HS, Chiu, YP, et al. Molecular investigation of two clusters of nosocomial bacteraemia caused by multiresistant Klebsiella pneumoniae using pulsed-field gel electrophoresis and infrequent-restriction-site PCR. J Hosp Infect 2000; 46:110–117.CrossRefGoogle Scholar
19.Rubin, LG, Sanchez, PL, Siegel, J, et al. Evaluation and treatment of neonates with suspected late-onset sepsis: a survey of neonatologists' practices. Pediatrics 2002; 110:e42.CrossRefGoogle ScholarPubMed