Hostname: page-component-8448b6f56d-wq2xx Total loading time: 0 Render date: 2024-04-19T06:52:40.868Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of Pulsed-Field Gel Electrophoresis in Infection Control Issues Concerning Burkholderia cepacia

Published online by Cambridge University Press:  02 January 2015

Adam Jenney ,
Lisa Liolios ,
Denis Spelman ,
Philip Russo ,
John Wilson ,
Steven Wesselingh  and
Tom Kotsimbos
Adam Jenney*
Affiliation:
Microbiology Department, The Alfred Hospital, Melbourne, Victoria, Australia Department of Microbiology and Immunology, The University of Melbourne, Melbourne, Victoria, Australia
Lisa Liolios
Affiliation:
Infectious Diseases Unit, The Alfred Hospital, Melbourne, Victoria, Australia
Denis Spelman
Affiliation:
Microbiology Department, The Alfred Hospital, Melbourne, Victoria, Australia Infectious Diseases Unit, The Alfred Hospital, Melbourne, Victoria, Australia
Philip Russo
Affiliation:
Infectious Diseases Unit, The Alfred Hospital, Melbourne, Victoria, Australia
John Wilson
Affiliation:
Respiratory Unit, The Alfred Hospital, Melbourne, Victoria, Australia
Steven Wesselingh
Affiliation:
Infectious Diseases Unit, The Alfred Hospital, Melbourne, Victoria, Australia Burnet Institute, Melbourne, Victoria, Australia
Tom Kotsimbos
Affiliation:
Respiratory Unit, The Alfred Hospital, Melbourne, Victoria, Australia
*
Microbiology Department, The Alfred Hospital, Commercial Road, Prahran, Victoria 3181, Australia
Get access Rights & Permissions [Opens in a new window]

Abstract

There was concern that nosocomial person-to-person transmission of Burkholderia cepacia had occurred when two patients with cystic fibrosis shared a bathroom. Pulsed-field gel electrophoresis demonstrated that the two isolates were unrelated. Subsequent testing of 34 stored isolates of B. cepacia demonstrated that no particular clone predominated in our hospital.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 24 , Issue 8 , August 2003 , pp. 624 - 626
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2003

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Isles, A, Macluskey, M, Corey, M, et al.Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J Pediatr 1984;104:206–210.CrossRefGoogle ScholarPubMed
2.Snell, GI, de Hoyos, A, Krajden, M, et al.Pseudomonas cepacia in lung transplant recipients with cystic fibrosis. Chest 1993;103:466–471.CrossRefGoogle ScholarPubMed
3.Paul, ML, Pegler, MAM, Benn, RAV. Molecular epidemiology of Burkholderia cepacia in two Australian cystic fibrosis centres. J Hosp Infect 1998;38:19–26.CrossRefGoogle ScholarPubMed
4.Govan, JRW. Infection control in cystic fibrosis. J R Soc Med 2000;93(suppl 38):40–45.Google ScholarPubMed
5.Cystic Fibrosis Australia. Infection Control and Hygiene Guidelines. Sydney: Cystic Fibrosis Australia; 2000.Google Scholar
6.Tenover, FC, Arbeit, RD, Goering, RV, et al.Interpreting chromosomal DNA-restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995;33:2233–2239.CrossRefGoogle ScholarPubMed
7.Steinbach, S, Sun, L, Jiang, R-Z, et al.Transmissibility of Pseudomonas cepacia infection in clinic patients and lung transplant recipients with cystic fibrosis. N Engl J Med 1994;331:981–987.CrossRefGoogle ScholarPubMed
8.Pitt, TL, Kaufmann, ME, Patel, PS. The characteristics and antibiotic susceptibility of Burkholderia (Pseudomonas) cepacia isolates from patients with cystic fibrosis in the United Kingdom and the Republic of Ireland. J Med Microbiol 1996;44:204–210.CrossRefGoogle Scholar
9.Holmes, A, Nolan, R, Taylor, R, et al.An epidemic of Burkholderia cepacia transmitted between patients with and without cystic fibrosis. J Infect Dis 1999;179:1197–1205.CrossRefGoogle ScholarPubMed
10.Segonds, C, Bingen, E, Couetdic, G, et al.Genotypic analysis of Burkholderia cepacia isolates from 13 French cystic fibrosis centres. J Clin Microbiol 1997;35:2055–2060.CrossRefGoogle Scholar