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Use of a severe acute respiratory coronavirus virus 2 (SARS-CoV-2) strand-specific assay to evaluate for prolonged viral replication >20 days from illness onset

Published online by Cambridge University Press:  29 June 2023

Jessica D. Ferguson*
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Ralph Tayyar
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Gustavo Contreras
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Melanie Kiener
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Alex N. Zimmet
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Caitlin A. Contag
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Guillermo Rodriguez Nava
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Lucy S. Tompkins
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
John Shepard
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Ayelet Rosenthal
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Aruna K. Subramanian
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Benjamin A. Pinsky
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
Jorge L. Salinas
Affiliation:
Stanford University School of Medicine and Stanford Hospital & Clinics, Stanford, California
*
Corresponding author: Jessica D. Ferguson; Email: jferg1@stanford.edu

Abstract

Severe acute respiratory coronavirus virus 2 (SARS-CoV-2) real-time reverse-transcription polymerase chain reaction (rRT-PCR) strand-specific assay can be used to identify active SARS-CoV-2 viral replication. We describe the characteristics of 337 hospitalized patients with at least 1 minus-strand SARS-CoV-2 assay performed >20 days after illness onset. This test is a novel tool to identify high-risk hospitalized patients with prolonged SARS-CoV-2 replication.

Type
Concise Communication
Copyright
© The Author(s), 2023. Published by Cambridge University Press on behalf of The Society for Healthcare Epidemiology of America

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Footnotes

PREVIOUS PRESENTATION. These data were presented in a poster presentation at IDWeek 2022, October 22, 2022, in Washington, DC.

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