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Molecular Epidemiology of Invasive Aspergillosis: Lessons Learned from an Outbreak Investigation in an Australian Hematology Unit

Published online by Cambridge University Press:  02 January 2015

Sarah E. Kidd
Affiliation:
Department of Medicine, Monash University, Melbourne, Victoria, Australia Infectious Diseases Unit, Alfred Hospital, Melbourne, Victoria, Australia MacFarlane Burnet Institute, Melbourne, Victoria, Australia
Li Min Ling
Affiliation:
Infectious Diseases Unit, Alfred Hospital, Melbourne, Victoria, Australia Infectious Diseases Unit, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia Infectious Diseases Department, Tan Tock Seng Hospital, Singapore
Wieland Meyer
Affiliation:
Centre for Infectious Diseases and Microbiology, Westmead Hospital, Sydney, New South Wales, Australia Department of Medicine, University of Sydney Western Clinical School, Westmead Millennium Institute, Sydney, New South Wales, Australia
C. Orla Morrissey
Affiliation:
Department of Medicine, Monash University, Melbourne, Victoria, Australia Infectious Diseases Unit, Alfred Hospital, Melbourne, Victoria, Australia MacFarlane Burnet Institute, Melbourne, Victoria, Australia
Sharon C. A. Chen
Affiliation:
Centre for Infectious Diseases and Microbiology, Westmead Hospital, Sydney, New South Wales, Australia Department of Medicine, University of Sydney Western Clinical School, Westmead Millennium Institute, Sydney, New South Wales, Australia
Monica A. Slavin*
Affiliation:
Infectious Diseases Unit, Alfred Hospital, Melbourne, Victoria, Australia Infectious Diseases Unit, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia Centre for Clinical Research Excellence in Infectious Diseases, Victorian Infectious Diseases Service, Melbourne, Victoria, Australia
*
Locked Bag 1, A'Beckett Street, Melbourne, VIC, 8006, Australia (monica.slavin@petermac.org)

Abstract

Suspected nosocomial Aspergillus fumigatus infections in an Australian hematology unit were investigated by molecular typing of clinical and environmental isolates using polymerase chain reaction fingerprinting, CSP typing, and multilocus microsatellite typing. Only multilocus microsatellite typing revealed that all isolates were genetically distinct. The selection of an appropriate typing method is essential for effective outbreak investigations.

Type
Concise Communications
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2009

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References

1.de Valk, HA, Klaassen, CH, Meis, JF. Molecular typing of Aspergillus species. Mycoses 2008;51:463476.CrossRefGoogle ScholarPubMed
2.Meyer, W, Marszewska, K, Amirmostofian, M, et al.Molecular typing of global isolates of Cryptococcus neoformans var. neoformans by polymerase chain reaction fingerprinting and randomly amplified polymorphic DNA— a pilot study to standardize techniques on which to base a detailed epidemiological survey. Electrophoresis 1999;20:17901799.3.0.CO;2-2>CrossRefGoogle Scholar
3.Balajee, SA, Tay, ST, Lasker, BA, Hurst, SF, Rooney, ARCharacterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America. Eukaryot Cell 2007;6:13921399.CrossRefGoogle ScholarPubMed
4.Klaassen, CH, de Valk, HA, Balajee, SA, Meis, JF. Utility of CSP typing to sub-type clinical Aspergillus fumigatus isolates and proposal for a new CSP type nomenclature. J Microbiol Methods 2009;77:292296.Google Scholar
5.de Valk, HA, Meis, JF, Curfs, IM, Muehlethaler, K, Mouton, JW, Klaassen, CH. Use of a novel panel of nine short tandem repeats for exact and high-resolution fingerprinting of Aspergillus fumigatus isolates. J Clin Microbiol 2005;43:41124120.CrossRefGoogle ScholarPubMed
6.White, TJ, Bruns, T, Lee, S, Taylor, J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, MA, Gelfand, DH, Sninsky, JJ, White, TJ, eds. PCR protocols—a guide to methods and applications. San Diego, CA: Academic Press; 1990:315322Google Scholar
7.Singh, A, Goering, RV, Simjee, S, Foley, SL, Zervos, MJ. Application of molecular techniques to the study of hospital infection. Clin Microbiol Rev 2006;19:512530.CrossRefGoogle Scholar
8.Bain, JM, Tavanti, A, Davidson, AD, et al.Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus. J Clin Microbiol 2007;45:14691477.Google Scholar
9.Balajee, SA, de Valk, HA, Lasker, BA, Meis, JF, Klaassen, CH. Utility of a microsatellite assay for identifying clonally related outbreak isolates of Aspergillus fumigatus. J Microbiol Methods 2008;73:252256.Google Scholar
10.de Valk, HA, Meis, JF, Bretagne, S, et al.Interlaboratory reproducibility of a microsatellite-based typing assay for Aspergillus fumigatus through the use of allelic ladders: proof of concept. Clin Microbiol Infect 2009;15:180187.CrossRefGoogle ScholarPubMed