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A Xenopus laevis creatine kinase isozyme (CK-III/III) expressed preferentially in larval striated muscle: cDNA sequence, developmental expression and subcellular immunolocalization

Published online by Cambridge University Press:  14 April 2009

Jacques Robert ,
Benjamin Barandun  and
Hans Rudolf Kobel
Jacques Robert
Affiliation:
Laboratoire de Génétique, Université de Genève, 154 bis route de Malagnou, CH-1224 Chêne-Bougeries, Geneva, Switzerland
Benjamin Barandun
Affiliation:
Laboratoire de Génétique, Université de Genève, 154 bis route de Malagnou, CH-1224 Chêne-Bougeries, Geneva, Switzerland
Hans Rudolf Kobel
Affiliation:
Laboratoire de Génétique, Université de Genève, 154 bis route de Malagnou, CH-1224 Chêne-Bougeries, Geneva, Switzerland

Summary

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A cDNA containing the nearly complete coding sequence of CK-III subunit of X. laevis was isolated, sequenced and further identified by comparing the tissue distribution of CK-III/III isozyme with that of its messenger. Comparison of CK-III deduced amino acid sequence with other CK sequences published reveals its close homology to M-CK subunits. Results using both cDNA probes and monoclonal antibodies specific for CK-III subunits indicate that the appearance and the accumulation of CK-III occur in parallel with myoblast differentiation. Moreover, subcellular immuno-histolocalization shows that CK-III/III isozyme is especially concentrated on larval myofibres at the level of A-bands.

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 1 , August 1991 , pp. 35 - 40
Copyright
Copyright © Cambridge University Press 1991

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