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Use of isozymes as chromosome markers in the isolation and characterization of wheat-barley chromosome addition lines

Published online by Cambridge University Press:  14 April 2009

Gary E. Hart ,
A. K. M. R. Islam  and
K. W. Shepherd
Gary E. Hart
Affiliation:
Agronomy Department, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia
A. K. M. R. Islam
Affiliation:
Agronomy Department, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia
K. W. Shepherd
Affiliation:
Agronomy Department, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia

Summary

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The alcohol dehydrogenase (ADH), glutamic oxaloacetic transaminase (GOT), aminopeptidase (AMP), endopeptidase (EP), and esterase (EST) zymogram phenotypes of Chinese Spring wheat, Betzes barley, Chinese Spring-Betzes heptaploids, and a number of presumptive Betzes chromosome additions to Chinese Spring were determined. It was found that four disomic chromosome addition lines could be distinguished from one another and from the other three possible lines on the basis of the zymogram phenotypes of these isozymes.

The structural gene Adh-H1 was located in Betzes chromosome 4, the genes Got-H2 and Amp-H1 in chromosome 6, and the gene Ep-H1 in chromosome 1. These gene locations provide evidence of homoeology between Betzes chromosomes 4, 6, and 1 and the Chinese Spring chromosomes of homoeologous groups 4, 6, and 7, respectively.

Type
Research Article
Information
Genetics Research , Volume 36 , Issue 3 , December 1980 , pp. 311 - 325
Copyright
Copyright © Cambridge University Press 1980

References

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