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The use of bacteriophages for differentiating plasmids of Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

V. Krishnapillai
V. Krishnapillai
Affiliation:
Department of Genetics, Monash University, Clayton 3168, Victoria, Australia

Summary

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Six bacteriophages have been used in the classification of 19 plasmids (antibiotic resistance-mediating R factors and FP sex factors which promote host chromosome transfer) of P. aeruginosa isolated in different geographical regions. On the basis of phage-plating responses on isogenic strains of bacteria differing only in the plasmids carried, five groups of plasmids were distinguishable. In general the groups could be correlated with their geographical origin although differences between plasmids from the same region were found. The unique phage-plating responses were also useful in establishing the possible identity of plasmids isolated from the same original strain and given different designations by independent investigators. The classification of the plasmids derived here on the basis of phage-plating responses could be correlated with classifications based upon other phenotypic characteristics described elsewhere. The nature of inhibition of plating of phages B39 and G101 by R18–1 and R18–3 respectively was shown to be due to interference with some aspect of intra-cellular phage replication rather than to plasmid-mediated restriction.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 3 , June 1974 , pp. 327 - 334
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience.CrossRefGoogle Scholar
Bannister, D. & Glover, S. W. (1968). Restriction and modification of bacteriophages by R+ strains of Escherichia coli K12. Biochemical & Biophysical Research Communications 30, 735–738.CrossRefGoogle Scholar
Black, W. A. & Girdwood, R. W. A. (1969). Carbenicillin resistance in Pseudomonas aeruginosa. British Medical Journal iv, 234.CrossRefGoogle Scholar
Bryan, L. E., Semaka, S. D., Van Den Elzen, H. M., Kinnear, J. E. & Whitehouse, R. L. S. (1973). Characteristics of R931 and other Pseudomonas aeruginosa R factors. Antimicrobial Agents & Chemotherapy 3, 625–637.CrossRefGoogle ScholarPubMed
Chabbert, Y. A., Scavizzi, M. P., Witchitz, J. L., Gerband, G. R. & Bouanchaud, D. H. (1972). Incompatibility groups and the classification of fi− resistance factors. Journal of Bacteriology 112, 666–675.CrossRefGoogle ScholarPubMed
Chandler, P. M. & Krishnapillai, V. (1974 a). Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors readily transferable into Enterobacteriaceae. Genetical Research (in the Press).Google ScholarPubMed
Chandler, P. M. & Krishnapillai, V. (1974 b). Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors transferable only within Pseudomonas aeruginosa. Genetical Research (in the Press).Google ScholarPubMed
Chandler, P. M. & Krishnapillai, V. (1974 c). Isolation and properties of recombination deficient mutants of Pseudomonas aeruginosa. Mutation Research 23, 15–23.CrossRefGoogle ScholarPubMed
Datta, N., Hedges, R. W., Shaw, E. J., Sykes, R. B. & Richmond, M. H. (1971). Properties of an R factor from Pseudomonas aeruginosa. Journal of Bacteriology 108, 1244–1249.CrossRefGoogle Scholar
Grinsted, J., Saunders, J. R., Ingram, L. C., Sykes, R. B. & Richmond, M. H. (1972) Properties of an R factor which originated in Pseudomonas aerucfinosa 1822. Journal of Bacteriology 110, 529–537.CrossRefGoogle Scholar
Hedges, R. W. (1972). Phenotypic characterisation of fi − R factors determining the restriction and modification of hspII specificity. Molecular & General Genetics 115, 225–233.CrossRefGoogle Scholar
Holloway, B. W. (1969). Genetics of Pseudomonas. Bacteriological Reviews 33, 419–443.CrossRefGoogle ScholarPubMed
Holloway, B. W. & Richmond, M. H. (1973). R factors used for genetic studies in strains of Pseudomonas aeruginosa and their origin. Genetical Research 21, 103–105.CrossRefGoogle Scholar
Ingram, L. C., Sykes, R. B., Grinsted, J., Saunders, J. R. & Richmond, M. H. (1972). A transmissible resistance element from a strain of Pseudomonas aeruginosa containing no detectable extrachromosomal DNA. Journal of General Microbiology 72, 269–279.CrossRefGoogle ScholarPubMed
Isaac, J. H. & Holloway, B. W. (1968). Control of pyrimidine biosynthesis in Pseudomonas aeruginosa. Journal of Bacteriology, 96, 1732–1741.CrossRefGoogle ScholarPubMed
Kawakami, Y., Mikoshiba, F., Nagasaki, S., Matsumoto, H. & Tazaki, T. (1972). Prevalence of Pseudomonas aeruginosa strains possessing R factor in a hospital. The Journal of Antibiotics 25, 607–609.CrossRefGoogle ScholarPubMed
Krishnapillai, V. (1971). A novel transducing phage: Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa. Molecular & General Genetics 114, 134–143.CrossRefGoogle Scholar
Lowbury, E. J. L., Kidson, A., Lilly, H. A., Ayliffe, G. A. J. & Jones, R. J. (1969). Sensitivity of Pseudomonas aeruginosa to antibiotics: Emergence of strains highly resistant to carbenicillin. Lancet ii, 448–452.CrossRefGoogle Scholar
Matsumoto, H. & Tazaki, T. (1973). FP5 factor, an undescribed sex factor of Pseudomonas aeruginosa. Japanese Journal of Microbiology 17, 409–417.CrossRefGoogle ScholarPubMed
Meyneil, E., Meynell, G. G. & Datta, N. (1968). Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriological Reviews 32, 55–83.CrossRefGoogle Scholar
Meynell, G. G. (1972). Bacterial Plasmids. London: Macmillan.Google Scholar
Mizobuchi, K., Anderson, G. C. & McCorquodale, D. J. (1971). Abortive infection by bacteriophage BF23 due to the colioin Ib factor. I. Genetic studies of nonrestricted and amber mutants of bacteriophage BF23. Genetics 68, 323–340.CrossRefGoogle Scholar
Morrison, T. G. & Malamy, M. H. (1971). T7 translational control mechanisms and their inhibition by F factors. Nature New Biology 231, 37–41.CrossRefGoogle Scholar
Moyer, R. W., Fu, A. S. & Szabo, G. (1972). Regulation of bacteriophage T5 development by Col I factors. Journal of Virology, 9, 804–812.CrossRefGoogle Scholar
Nishioka, T. & Ozeki, H. (1968). Early abortive lysis by phage BF23 in Escherichia coli K12 carrying the colicin Ib factor. Journal of Virology 2, 1249–1254.CrossRefGoogle Scholar
Olsen, R. H. & Shipley, P. (1973). Host range and properties of the Pseudomonas aeruginosa R factor R1822. Journal of Bacteriology 113, 772–780.CrossRefGoogle ScholarPubMed
Pemberton, J. M. & Holloway, B. W. (1973). A new sex factor of Pseudomonas aeruginosa. Genetical Research 21, 263–272.CrossRefGoogle ScholarPubMed
Pitton, J. S. & Anderson, E. S. (1970). The inhibitory action of transfer factors on lysis of Escherichia coli K12 by phages μ2 and φ2. Genetical Research 16, 215–224.CrossRefGoogle Scholar
Saunders, J. R. & Grinsted, J. (1972). Properties of RP4, an R factor which originated in Pseudomonas aeruginosa S8. Journal of Bacteriology 112, 690–696.CrossRefGoogle Scholar
Stanisich, V. A. (1974 a). Interaction between an R factor and an element conferring resistance to mercuric ions in Pseudomonas aeruginosa. Molecular and General Genetics 128, 201–212.CrossRefGoogle Scholar
Stanisich, V. A. (1974 b). Male specific bacteriophages of Pseudomonas aeruginosa: their use in determining similarities between FP and RP plasmids. Journal of General Microbiology (in the Press).CrossRefGoogle Scholar
Stanisich, V. A. & Holloway, B. W. (1971). Chromosome transfer in Pseudomonas aeruginosa mediated by R factors. Genetical Research 17, 169–172.CrossRefGoogle ScholarPubMed
Watanabe, T., Takano, T., Arae, T., Nishida, H. & Sato, S. (1966). Episome-mediated transfer of drug resistance in Enterobacteriaceae X. Restriction and modification of phages by fi− R factors. Journal of Bacteriology 92, 477–486.CrossRefGoogle ScholarPubMed