Hostname: page-component-76fb5796d-dfsvx Total loading time: 0 Render date: 2024-04-27T02:37:02.941Z Has data issue: false hasContentIssue false
  • English
  • Français

Three ENU-induced alleles of the murine quaking locus are recessive embryonic lethal mutations

Published online by Cambridge University Press:  14 April 2009

Monica J. Justice  and
Vernon C. Bode
Monica J. Justice
Affiliation:
Kansas State University, Division of Biology, Ackert Hall, Manhattan, Kansas 66506, USA
Vernon C. Bode
Affiliation:
Kansas State University, Division of Biology, Ackert Hall, Manhattan, Kansas 66506, USA
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The quaking (qk) locus on mouse chromosome 17 has been defined by a single viable quaking allele. Three new alleles of quaking were selected after ENU mutagenesis by their failure to complement the quaking phenotype. The qkk2 allele was induced on wild-type chromatin and the qkkt1 and qkkt4 alleles were induced on t-chromatin. Each is a recessive embryonic lethal mutation. They fail to complement each other and are not complemented by the deletion, TtOrl. Homozygotes and hemizygotes die at 8–9·5 days gestation, but not at a single precise time. Because the classical quaking mutation complements the lethality of these new alleles, but they fail to complement its quaking phenotype (myelination defect), we conclude that the quaking+ function is required for embryonic survival as well as for myelination.

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 2 , April 1988 , pp. 95 - 102
Copyright
Copyright © Cambridge University Press 1988

References

Baumann, N. A., Bourre, J. M., Jacque, C. & Pollett, S. (1972). Genetic disorders of myelination. In Lipids, Malnutrition and the Developing Brain (ed. Elliott, K. and Knight, J.). Elsevier, Amsterdam.Google Scholar
Bennett, W., Gall, A. M., Southard, J. L. & Sidman, R. L. (1971). Abnormal spermiogenesis in quaking, a myelin deficient mutant mouse. Biology of Reproduction 5, 30–58.CrossRefGoogle ScholarPubMed
Bode, V. C. (1984). Ethylnitrosourea mutagenesis and the isolation of mutant alleles for specific genes located in the t region of mouse chromosome 17. Genetics 108, 457–470.Google Scholar
Bourre, J.-M.Boiron, F., Cassagne, C., Dumont, O., Leterrier, F., Metzger, H. & Viret, J. (1986). Biochemical and physicochemical determinations in a premyelin fraction obtained by zonal centrifugation in normal mouse and in dysmyelinating mutants (quaking, shiverer, and myelin-deficient). Neurochemical Pathology 4, 29–42.Google Scholar
Fox, H. S., Martin, G. R., Lyon, M. F., Hermann, B., Frischauf, A.-M., Lerarch, H. & Silver, L. M. (1985). Molecular probes define different regions of the mouse t-complex. Cell 40, 63–69.Google Scholar
Justice, M. J. & Bode, V. C. (1986). Induction of new mutations in a mouse t-haplotype using ethylnitrosourea mutagenesis. Genetical Research 47, 187–192.Google Scholar
Mann, E. A., Silver, L. M. & Elliott, R. W. (1986). Genetic analysis of a mouse t complex locus that is homologous to a kidney cDNA clone. Genetics 114, 993–1006.Google Scholar
Popp, R. A., Bailiff, E. G., Skow, L. C., Johnson, F. M. & Lewis, S. E. (1983). Analysis of a mouse α-globin gene mutation induced by ethylnitrosourea. Genetics 105, 157–167.Google Scholar
Peters, J., Andrews, S. J., Loutit, J. F. & Glegg, J. B. (1985). A mouse β-globin mutant that is an exact model of hemoglobin Rainier in man. Genetics 110, 709–721.Google Scholar
Russell, W. L. (1951). X-ray-induced mutations in mice. Cold Spring Harbor Symposia on Quantitative Biology 16, 327–335.CrossRefGoogle ScholarPubMed
Russell, W. L. (1982). Factors affecting mutagenicity of ethylnitrosourea in the mouse specific-locus test and their bearing on risk estimation. In Environmental Mutagens and Carcinogens (ed. Sugimura, T., Kondo, S. and Takebe, H.), pp. 59–70. New York: Alan R. Liss.Google Scholar
Russell, W. L., Kelly, E. M., Hunsicker, P. R., Bangam, J. W., Maddux, C. & Phipps, E. L. (1979). Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse. Proceedings of the National Academy of Sciences, U.S.A. 76, 5818–5819.CrossRefGoogle ScholarPubMed
Samorajski, T., Friede, R. L. & Reimer, P. R. (1970). Hypomyelination in the quaking mouse. A model for the analysis of disturbed myelin formation. Journal of Neuropathology and Experimental Neurology 29, 507–523.Google Scholar
Shedlovsky, A., Guenet, J.-L., Johnson, L. L. & Dove, , William, F. (1986). Induction of recessive lethal mutations in the T/t-H-2 region of the mouse genome by a point mutagen. Genetical Research 47, 135–142.Google Scholar
Sidman, R. L., Dickie, M. M. & Appel, S. H. (1964). Mutant mice (quaking and jimpy) with deficient myelination in the central nervous system. Science 144, 309–311.Google Scholar
Silver, L. M., Lukralle, D. & Garrels, J. I. (1983). Torl is a novel, variant form of mouse chromosome 17 with a deletion in a partial t-haplotype. Nature 301, 422–424.CrossRefGoogle Scholar
Theiler, K. (1972). The House Mouse. New York: Springer-Verlag.Google Scholar
Vogel, E. & Natarjan, A. T. (1979). The relation between reaction kinetics and mutagenic action of mono-functional alkylating agents in higher eukaryotic systems. 1. Recessive lethal mutations and translocations in Drosophila. Mutation Research 62, 51–100.CrossRefGoogle ScholarPubMed