Hostname: page-component-848d4c4894-ndmmz Total loading time: 0 Render date: 2024-05-03T13:15:10.164Z Has data issue: false hasContentIssue false
  • English
  • Français

Survey of genomic repeat sequence-PCRs that detect differences between inbred mouse strains

Published online by Cambridge University Press:  14 April 2009

Philip A. Wood  and
Doug A. Hamm
Philip A. Wood*
Affiliation:
Department of Comparative Medicine, Schools of Medicine and Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA
Doug A. Hamm
Affiliation:
Department of Comparative Medicine, Schools of Medicine and Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA
*
* Corresponding authors.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

We have developed molecular markers that distinguish between several inbred and congenic mouse strains using polymerase chain reaction (PCR) amplification of genomic DNA repeat sequences. Mouse genomic DNA, digested with four base recognition site-restriction endonucleases, was amplified by PCR using primers for the following repeat sequences: Bl (Alu homolog), LINE, LLR3, IAP, human Alu and myoglobin. Amplification products analysed by agarose gel electrophoresis and stained with ethidium bromide produced unique DNA fragments, some of which are specific for each of 12 strains tested. This method can be used for molecular analysis of the mouse genome, including genetic monitoring.

Type
Short Paper
Information
Genetics Research , Volume 65 , Issue 2 , April 1995 , pp. 151 - 155
Copyright
Copyright © Cambridge University Press 1995

References

Atchley, W. R., & Fitch, W. M., (1991). Gene trees and the origins of inbred strains of mice. Science 254, 554–559.CrossRefGoogle ScholarPubMed
Fanning, T. G., (1983). Size and structure of the highly repetitive BAM HI element in mice. Nucleic Acids Research 11, 5073–5091.CrossRefGoogle ScholarPubMed
Heller, D. L., Gianola, K. M., & Leinwand, L. A., (1988). A highly conserved mouse gene with a propensity to form pseudogenes in mammals. Molecular and Cellular Biology 8, 2797–2803.Google ScholarPubMed
Herman, G. E., Nadeau, J. H., & Hardies, S. C., (1992). Dispersed repetitive elements in mouse genome analysis. Mammalian Genome 2, 207–217.CrossRefGoogle ScholarPubMed
Hinsdale, M. E., Kelly, C. L., & Wood, P. A., (1993). Null allele at bcd-1 locus in BALB/cByJ mice is due to a deletion in the short-chain acyl-CoA dehydrogenase gene and results in missplicing of mRNA. Genomics 16, 605–611.CrossRefGoogle ScholarPubMed
Jeffreys, A. J., Wilson, V., Kelly, R., Taylor, B. A., & Bulfield, G., (1987). Mouse DNA fingerprint analysis of chromosome localization and germ-line stability of hypervariable loci in recombinant inbred strains. Nucleic Acids Research 15, 2823–2836.CrossRefGoogle Scholar
Jelinek, W. R., & Schmid, C. W., (1982). Repetitive sequences in eukaryotic DNA and their expression. Annual Review of Biochemistry 51, 813–844.CrossRefGoogle ScholarPubMed
Krayev, A. S., Kramerov, D. A., Skryabin, K. G., Ryskov, A. P., Bayev, A. A., & Georgiev, G. P., (1980). The nucleotide sequence of the ubiquitous repetitive DNA sequence Bl complementary to the most abundant class Genomic PCR patterns of mice 155 of mouse fold-back RNA. Nucleic Acids Research 8, 1201–1215.CrossRefGoogle Scholar
Nadeau, J. H., Bedigian, H. G., Bouchard, G., Denial, T., Kosowsky, M., Norberg, R., Pugh, S., Sargeant, E., Turner, R., & Paigen, B., (1992). Multilocus markers for mouse genome analysis: PCR amplification based on single primers of arbitrary nucleotide sequence. Mammalian Genome 3, 55–64.CrossRefGoogle ScholarPubMed
Nelson, D. L., Ledbetter, S. A., Corbo, L., Victoria, M. F., Ramirez-Solis, R., Webster, T. D., Ledbetter, D. H., & Caskey, C. T., (1989). Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources. Proceedings of the National Academy of Sciences, USA 86, 6686–6690.CrossRefGoogle ScholarPubMed
Weller, P., Jeffreys, A. J., Wilson, V., & Blanchetot, A., (1984). Organization of the human myoglobin gene. EMBO Journal 3, 439–446.CrossRefGoogle ScholarPubMed
Wood, P. A., Hinsdale, M. E., & Kelly, C. L., (1993). Molecular detection of the Bcd-l null allele in BALB/cByJ mice by polymerase chain reaction: a simple assay for genetic monitoring. Mouse Genome 91, 342–344.Google Scholar
Woodward, S. R., Sudweeks, J., & Teuscher, C., (1992). Random sequence oligonucleotide primers detect polymorphic DNA products which segregate in inbred strains of mice. Mammalian Genome 3, 73–78.CrossRefGoogle ScholarPubMed
Ymer, S., Tucker, W. Q. J., Campbell, H. D., & Young, I. G., (1986). Nucleotide sequence of the intracisternal Aparticle genome inserted 5′ to the interleukin-3 gene of the leukaemia cell line WEHI-3B. Nucleic Acids Research 14, 5901–5918.CrossRefGoogle Scholar
Zechner, R., Newman, T. C., Steiner, E., & Breslow, J. L., (1991). The structure of the mouse lipoprotein lipase gene: a B1 repetitive element is inserted into the 3′ untranslated region of the mRNA. Genomics 11, 62–76.CrossRefGoogle ScholarPubMed