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SHORT PAPER P element excision in Drosophila is stimulated by gamma-irradiation in transient embryonic assays

Published online by Cambridge University Press:  01 August 1997

ALFRED M. HANDLER  and
SHEILACHU P. GOMEZ
ALFRED M. HANDLER
Affiliation:
Center for Medical, Agricultural, and Veterinary Entomology, Agricultural Research Service, US Department of Agriculture, 1700 S.W. 23rd Drive, Gainesville, FL 32608, USA
SHEILACHU P. GOMEZ
Affiliation:
Center for Medical, Agricultural, and Veterinary Entomology, Agricultural Research Service, US Department of Agriculture, 1700 S.W. 23rd Drive, Gainesville, FL 32608, USA

Abstract

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The influence of gamma-irradiation on P element excision and excision-site repair mechanisms was directly tested by embryonic somatic excision assays. Preblastoderm P[ry+, Δ2–3](99B) embryos, having a stable source of somatically active P transposase, were irradiated previous to injection with P excision indicator plasmids. Frequencies of precise or nearly precise P excision increased with gamma-ray doses ranging from 0·5 to 3·5 Gy. Higher doses resulted in frequencies close to that in unirradiated embryos, though considerable embryonic lethality was also evident at these doses. A direct positive interaction between gamma-irradiation and P element activity is concluded.

Type
Brief Report
Information
Genetics Research , Volume 70 , Issue 1 , August 1997 , pp. 75 - 78
Copyright
© 1997 Cambridge University Press