Hostname: page-component-848d4c4894-sjtt6 Total loading time: 0 Render date: 2024-07-05T09:31:31.307Z Has data issue: false hasContentIssue false

R.SmaI cleavage map of the transfer region of the E. coli K12 sex factor F

Published online by Cambridge University Press:  14 April 2009

Neil Willetts
Affiliation:
Department of Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, Scotland
Gillian Johnson
Affiliation:
Department of Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, Scotland

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A map of the seventeen R.SmaI sites within the 33·4 kb transfer region of plasmid F has been obtained. The number of R.SmaI sites contrasts sharply with the few sites for other restriction endonucleases with hexanucleotide specificity. Location of the R.SmaI sites in γtra transducing phages allowed physical location of lambda insertions into the traB, traF and traH genes.

Type
Short papers
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

Achtman, M., Skurray, R. A., Thompson, R., Helmuth, R., Hall, S., Beutin, L. & Clark, A. J. (1978). Assignment of tra cistrons to EcoRI fragments of F sex factor DNA. Journal of Bacteriology 133, 13831392.CrossRefGoogle ScholarPubMed
Bolivar, F. (1978). Construction and characterisation of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4, 121136.CrossRefGoogle ScholarPubMed
Clark, A. J., Skurray, R. A., Crisona, N. J. & Nagaishi, H. (1976). Use of molecular cloning in the genetic and functional analyses of the plasmid F. In Molecular Mechumisms in the Control of Gene Expression, pp. 565579. New York: Academic Press.CrossRefGoogle Scholar
Humphreys, G. O., Willshaw, G. A. & Anderson, E. S. (1975). A simple method for the preparation of large quantities of pure plasmid DNA. Biochimica et biophysica acta 383, 457463.CrossRefGoogle ScholarPubMed
McIntire, S. & Willetts, N. S. (1979). Transfer-deficient cointegrates of Flac and lambda prophage. Molecular and General Genetics (submitted for publication).Google Scholar
McParland, R. H., Brown, L. R. & Pearson, G. D. (1976). Cleavage of lambda DNA by a site-specific endonuclease from Serratia marcescens. Journal of Virology 19, 10061011.CrossRefGoogle ScholarPubMed
Ohtsubo, H. & Ohtsubo, E. (1977). Repeated DNA sequences in plasmids, phages, and bacterial chromosomes. In DNA Insertion Elements Plasmids and Episomes, (ed. Bukhari, A. I., Shapiro, J. A. and Adhya, S.), pp. 4964. Cold Spring Harbor Laboratory, New York.Google Scholar
Skurray, R. A., Nagaishi, H. & Clark, A. J. (1978). Construction and BamHI analysis of chimeric plasmids containing EcoRI DNA fragments of the F sex factor. Plasmid 1, 174186.CrossRefGoogle Scholar
Thompson, R. & Achtman, M. (1978). The control region of the F sex factor DNA transfer cistrons: restriction mapping and DNA cloning. Molecular and General Genetics 165, 295304.CrossRefGoogle Scholar
Willetts, N. S. (1977). The transcriptional control of fertility in F-like plasmids. Journal of Molecular Biology 112, 141148.CrossRefGoogle ScholarPubMed
Willetts, N. S. & McIntire, S. (1978). Isolation and characterisation of λtra transducing phages from EDFL223 (Flac traB:: EDλ4). Journal of Molecular Biology, 126, 525549.CrossRefGoogle ScholarPubMed