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Restricted trandsuction by bacteriophage P22 in Salmonella typhimurium

Published online by Cambridge University Press:  14 April 2009

P. F. Smith-Keary
P. F. Smith-Keary
Affiliation:
Department of Genetics, Trinity College, Dublin

Extract

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1. In the transduction pro-401 (×) + some of the transductants are surrounded by several hundred small wild-type satellite colonies; these transductants spontaneously release phage which transduces pro-401 to wild-type at high frequency (HFT phage).

2. When the HFT phage is used to infect pro-401 at very low multiplicities of infection, most of the transductants are defective lysogens and segregate proline-requiring phage-sensitive derivatives; these transductants are apparently heterogenotes. At higher multiplicities of infection, or with lysogenic recipients, a higher proportion of satellited transductants is found.

3. The HFT phage preparations transduce only the proline region of the donor genome.

4. The existence is inferred of a defective P22 particle specifically incorporating the proline region of the Salmonella chromosome; these defective particles can establish themselves as prophage and confer immunity upon the infected cell, but are unable to replicate unless a normal prophage is also present. Satellited transductants are lysogenic both for a normal and defective (proline region carrying) phage, and so on lysis release transducing phage.

5. This system is compared with the λdg-gal and P1-dl-lac systems in E. coli.

Type
Research Article
Information
Genetics Research , Volume 8 , Issue 1 , August 1966 , pp. 73 - 82
Copyright
Copyright © Cambridge University Press 1966

References

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