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Repressor-minus and operator-constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC

Published online by Cambridge University Press:  14 April 2009

Elinor Meynell  and
Marylyn Cooke
Elinor Meynell
Affiliation:
Department of Microbiology and Guinness–Lister Research Unit, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, S. W. 1
Marylyn Cooke
Affiliation:
Department of Microbiology and Guinness–Lister Research Unit, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, S. W. 1

Extract

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Certain drd mutants of fi+ R factors, when carried by strain HfrC, allowed polarized transfer of the Hfr chromosome to occur at the normal rate. These mutants were independently shown to be repressor-sensitive and so owed their de-repression to failure to produce represser (i−). With other drd mutants, independently shown to be repressor-insensitive (0c), the rate of polarized chromosome transfer was as low as with the wild type and only R pili were produced by the HfrC+ bacteria. These R factors, therefore, continued to produce repressor and the donor behaviour of an Hfr strain depends on functioning of the integrated F.

Type
Research Article
Information
Genetics Research , Volume 14 , Issue 3 , December 1969 , pp. 309 - 313
Copyright
Copyright © Cambridge University Press 1969

References

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