Hostname: page-component-7479d7b7d-qlrfm Total loading time: 0 Render date: 2024-07-12T21:29:41.900Z Has data issue: false hasContentIssue false
  • English
  • Français

The relation between virus-like particles and R bodies of Paramecium aurelia

Published online by Cambridge University Press:  14 April 2009

John R. Preer Jr  and
Artur Jurand
John R. Preer Jr
Affiliation:
Institute of Animal Genetics, West Mains Rood, Edinburgh 9
Artur Jurand
Affiliation:
Institute of Animal Genetics, West Mains Rood, Edinburgh 9

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Virus-like particles can be seen in sections of kappa of stocks 7 and 562. The virus-like particles are specifically associated with R bodies and are rare or absent in kappa particles lacking R bodies. This observation clearly links the virus-like particles with kappa's toxic activity; it also supports the suggestion that kappa is infected with a lysogenic virus whose induction results in the production of the R body and viruses. Many ‘unfilled’ virus-like particles are present in whole kappa particles. Wound R bodies of stock 7 are surrounded by a sheath, whose breakage may provide the mechanism which causes the unrolling of the R body. One edge of the R body ribbon stains more intensely than the other.

Type
Research Article
Information
Genetics Research , Volume 12 , Issue 3 , December 1968 , pp. 331 - 340
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Afzelius, B. A. (1962). Chemical fixatives for electron microscopy. In Interpretations of Ultrastructure, pp. 1–19. Editor: Harris, R. J. C.. Academic Press: New York and London.Google Scholar
Anderson, T. F., Preer, J. R., Preer, L. B. & Bray, M. (1964). Studies on killing particles from Paramecium: the structure of refractile bodies from kappa particles. J. Microscopie 3, 395–402.Google Scholar
Dippell, R. V. (1958). The fine structure of kappa in killer stock 51 of Paramecium aurelia. Preliminary observations. J. biophys. biochem. Cytol. 4, 125–128.CrossRefGoogle ScholarPubMed
Dryl, S. (1959). Antigenic transformation in Paramecium aurelia after homologous anti-serum treatment during autogamy and conjugation. J. Protozool. 6 (suppl.), 25.Google Scholar
Jurand, A. & Ireland, M. J. (1965). A slow rotary shaker for embedding in viscous media. Stain Technol. 40, 233–234.CrossRefGoogle ScholarPubMed
Jurand, A. & Preer, L. B. (1968). Ultrastructure of flagellated lambda symbionts in Paramecium aurelia. J. gen. Microbiol. (in the Press).CrossRefGoogle ScholarPubMed
Morgan, C., Rose, H. M. & Moore, D. H. (1957). An evaluation of host cell changes accompanying viral multiplication as observed in the electron microscope. Ann. N.Y. Acad. Sci. 68, 302–323.CrossRefGoogle ScholarPubMed
Mueller, J. A. (1962). Induced physiological and morphological changes in the B particle and R body from killer paramecia. J. Protozool. 9, 26.Google Scholar
Mueller, J. A. (1963). Separation of kappa particles with infective activity from those with killing activity and identification of the infective particles in Paramecium aurelia. Expl Cell Res. 30, 492–508.CrossRefGoogle Scholar
Mueller, J. A. (1965). Vitally stained kappa in Paramecium aurelia. J. Exptl Zool. 160, 369–372.CrossRefGoogle ScholarPubMed
Preer, J. R. (1968. Genetics of the Protozoa. In Research in Protozoology, vol. 3, pp. 139–288. Ed. Chen, T. T.. Oxford: Pergamon Press.Google Scholar
Preer, J. R., Hufnagel, L. A. & Preer, L. B. (1966). Structure and behaviour of ‘R’ bodies from killer paramecia. J. Ultrastruct. Res. 15, 131–143.CrossRefGoogle ScholarPubMed
Preer, J. R. & Preer, L. B. (1967). Virus-like bodies in killer paramecia. Proc. natn. Acad. Sci. U.S.A. 58, 1774–1781.CrossRefGoogle ScholarPubMed
Preer, J. R., Siegel, R. W. & Stark, P. S. (1953). The relationship between kappa and paramecin in Paramecium aurelia. Proc. natn. Acad. Sci. U.S.A. 39, 1228–1233.CrossRefGoogle ScholarPubMed
Preer, J. R. & Stark, P. (1953). Cytological observations on the cytoplasmic factor ‘kappa’ in Paramecium aurelia. Expl Cell Res. 5, 478–491.CrossRefGoogle ScholarPubMed
Preer, L. B. & Preer, J. R. (1964). Killing activity from lysed kappa particles of Paramecium. Genet. Res. 5, 230–239.CrossRefGoogle Scholar
Rudenberg, F. H. (1962). Electron microscopic observations of kappa in Paramecium aurelia. Texas Reports on Biology and Medicine 20, 105–112.Google Scholar
Smith, J. E. (1961). Purification of kappa particles of Paramecium aurelia, stock 51. Am. Zool. 1, 390.Google Scholar
Sonneborn, T. M. (1959). Kappa and related particles in Paramecium. Adv. in Virus Res. 6, 229–356.CrossRefGoogle Scholar
Woods, D. R. & Bevan, E. A. (1968). Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J. gen. Microbiol. 51, 115–126.CrossRefGoogle ScholarPubMed
Wrischer, M. (1968). Cytoplasmatische Einschlüsse in virusinfizierten Bohnenblättern. Z. Naturforsch. 236, 80–82.CrossRefGoogle Scholar