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Recombination frequency and wild-type ancestry in linkage group I of Neurospora crassa

Published online by Cambridge University Press:  14 April 2009

Stella Lavigne  and
L. C. Frost
Stella Lavigne
Affiliation:
Genetics Laboratory, Department of Botany, University of Bristol
L. C. Frost
Affiliation:
Genetics Laboratory, Department of Botany, University of Bristol

Extract

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A selection of single and double mutant strains was backcrossed repetitively at least four times to each of the wild-types Abbott 4A, Abbott 12a and Lindegren 1A, and then re-isolated. Twelve crisp progeny isolated from the fifth backcross to Abbott 4A were heterogeneous for recombination frequency in the interval between cr and me-6 while thirteen crisp progeny from the fifth backcross to Lindegren 1A were homogeneous for recombination frequency in this interval. Thirteen crisp strains from the fifth backcross to Abbott 12a were homogeneous for recombination frequency between cr and nic-1 loci. However, mutant strains which had been backcrossed independently to Lindegren 1A were heterogeneous for recombination frequency in the intervals cr-aur and aur-nic-1.

In general, recombination frequencies in crosses between strains of the same wild-type ancestry including Abbott 4 and Abbott 12 were significantly higher than those in crosses of Lindegren × Abbott 4 or Lindegren × Abbott 12 ancestry but exceptions were found. Recombination frequencies between the same markers usually, but not always, increased on inbreeding and the changes in frequency were non-uniform in the marked region in some crosses.

The limitations of backcrossing as a means of transferring a mutant marker into a wild-type genome were discussed. It was concluded that inducing marker mutations anew in a given wild strain, preferably Lindegren 1A, might be more successful in obtaining constancy of map distance.

Type
Research Article
Information
Genetics Research , Volume 5 , Issue 3 , November 1964 , pp. 366 - 378
Copyright
Copyright © Cambridge University Press 1964

References

REFERENCES

Barratt, R. W. (1954). A word of caution: Genetic and cytological effects of Abbott stocks in Neurospora crassa. Microbial Genet. Bull. 11, 5–6.Google Scholar
Barratt, R. W., Newmeyer, D., Perkins, D. D. & Garnjobst, L. (1954). Map construction in Neurospora crassa. Advanc. Genet. 6, 1–93.CrossRefGoogle ScholarPubMed
Beadle, G. W. & Tatum, E. L. (1945). Neurospora. II. Methods of producing and detecting mutations concerned with nutritional requirements. Amer. J. Bot. 32, 678–685.Google Scholar
Emerson, S. & Cushing, J. E. (1946). Altered sulfonamide antagonism in Neurospora. Fed. Proc. 5, 379–389.Google ScholarPubMed
Frost, L. C. (1955 a). A possible interpretation of the cytogenetic effects of Abbott stocks in Neurospora crassa. Microbial Genet. Bull. 12, 7–9.Google Scholar
Frost, L. C. (1955 b). The genetics of some wild type and mutant strains of Neurospora crassa. Ph.D. Thesis, Cambridge University.Google Scholar
Frost, L. C. (1961). Heterogeneity in recombination frequencies in Neurospora crassa. Genet. Res. 2, 43–62.CrossRefGoogle Scholar
Lavigne, S. (1962). A selective plating technique for determining recombination frequency between crisp and linked mutant loci. Neurospora Newsletter, 2, 20.Google Scholar
Lindegren, C. C. (1933). The genetics of Neurospora III. Pure bred stocks and crossing-over in N. crassa. Bull. Torrey bot. Cl. 60, 133–154.CrossRefGoogle Scholar
Perkins, D. D. (1959). New markers and multiple point linkage data in Neurospora. Genetics, 44, 1185–1208.CrossRefGoogle ScholarPubMed
Prakash, V. (1963). Construction of multiple centromere marker strains in Neurospora crassa. Neurospora Newsletter, 3, 12–13.Google Scholar
Rifaat, O. M. (1958). A possible inversion in the mating-type chromosome of Neurospora crassa. Genetica, 29, 193–205.CrossRefGoogle ScholarPubMed
Stadler, D. R. (1956 a). Heritable factors influencing crossing over frequency in Neurospora. Microbiol Genet. Bull. 13, 32–34.Google Scholar
Stadler, D. R. (1956 b). A map of linkage group VT of Neurospora crassa. Genetics, 41, 528–543.CrossRefGoogle Scholar
Stadler, D. R. & Towe, A. M. (1962). Genetic factors influencing crossing over in Neurospora. Genetics, 47, 839–846.CrossRefGoogle ScholarPubMed
Towe, A. M. (1958). Factors influencing crossing over in Neurospora. Microbial Genet. Bull. 16, 31–32.Google Scholar
Westergaard, N. & Mitchell, H. K. (1947). Neurospora. V. A synthetic medium favouring sexual reproduction. Amer. J. Bot. 34, 573–577.CrossRefGoogle Scholar