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The properties of a temperate bacteriophage Wφ isolated from Escherichia coli strain W

Published online by Cambridge University Press:  14 April 2009

S. W. Glover  and
G. Kerszman
S. W. Glover
Affiliation:
Medical Research Council, Microbial Genetics Research Unit, Hammersmith Hospital, Ducane Road, London, W.12
G. Kerszman
Affiliation:
Medical Research Council, Microbial Genetics Research Unit, Hammersmith Hospital, Ducane Road, London, W.12

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Escherichia coli strain W was found to be lysogenic for a temperate phage Wφ. This phage, which plates on E. coli C, forms λ-like plaques 2–3 mm. diameter with turbid centres. It is serologically unrelated to λ but is closely related to P2 which it resembles in the electron microscope. Its buoyant density in CsCl has been measured and it is different from λ but similar to P2. E. coli C made lysogenic for Wφ restricts the growth of λ, and elsewhere (Kerszman, Glover & Aronovitch, 1967) it has been shown that the DNA of phage λ is degraded shortly after infection of bacteria lysogenic for Wφ. A mutant of Wφ has been isolated which has lost the property of restricting the growth of λ.

Type
Short Papers
Information
Genetics Research , Volume 9 , Issue 1 , February 1967 , pp. 135 - 139
Copyright
Copyright © Cambridge University Press 1967

References

REFERENCES

Adams, M. H. (1950). Methods for the study of bacterial viruses. In Methods in Medical Besearch (Comroe, J. H., ed.), vol. 2, pp. 1–73. Chicago: The Year Book Publishers, Inc.Google Scholar
Appleyard, R. K. (1954). Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K 12. Genetics, 39, 440–452.CrossRefGoogle Scholar
Arber, W. (1960). Polylysogeny for bacteriophage lambda. Virology, 11, 250–272.CrossRefGoogle Scholar
Arber, W. & Dussoix, D. (1962). Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage A. J. molec. Bid. 5, 18–36.CrossRefGoogle Scholar
Bertani, G. (1958). Lysogeny. Adv. Virus Res. 5, 151–193.CrossRefGoogle ScholarPubMed
Bertani, G. & Weigle, J. J. (1953). Host controlled variation in bacterial viruses. J. Bad. 65, 113–121.Google ScholarPubMed
Colson, C., Glover, S. W., Symonds, N. D. & Stacey, K. A. (1965). The location of the genes for host controlled modification and restriction in Escherichia coli K 12. Genetics, 52, 1043–1050.CrossRefGoogle ScholarPubMed
Davis, B. D. (1950). Studies on nutritionally deficient bacterial mutants isolated by means of penicillin. Experientia, 6, 41–50.CrossRefGoogle Scholar
Glover, S. W. (1962). Valine resistant mutants of Escherichia coli K-12. Genet. Res. 3, 448–460.CrossRefGoogle Scholar
Glover, S. W. & Arnovitch, J. (1967). Mutants of bacteriophage lambda able to grow on the restricting host Escherichia coli strain W. Genet. Res. 9, 129–133.CrossRefGoogle Scholar
Jacob, F. & Wollman, E. L. (1954). Etude génétique d'un bactériophage tempéré d'Escherichia coli. I. Le système gene'tique du bactériophage λ. Annls Inst. Pasteur, Paris, 87, 653–673.Google Scholar
Kerszman, G., Glover, S. W. & Arnovitch, J. (1967). The restriction of phage λ in Escherichia coli strain W. (in press).Google Scholar
Lennox, E. S. (1955). Transduction of linked genetic characters of the host by bacteriophage Pl. Virology, 1, 190–206.CrossRefGoogle Scholar
Pizer, L. I., Miovic, M. & Pylkas, L. (1967). The effects of prophage W on the propagation of T2 and T4. J. Bact. (in press).Google Scholar