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Penicillinase synthesis in Staphylococcus aureus: the effect of inducer and of the cellular location of the genetic determinants on the amount of enzyme formed

Published online by Cambridge University Press:  14 April 2009

Elizabeth H. Asheshov ,
Jennifer M. Woods  and
K. G. H. Dyke
Elizabeth H. Asheshov
Affiliation:
Cross-Infection Reference Laboratory, Colindale Avenue, London, N.W.9, and Department of Microbiology, University of Oxford
Jennifer M. Woods
Affiliation:
Cross-Infection Reference Laboratory, Colindale Avenue, London, N.W.9, and Department of Microbiology, University of Oxford
K. G. H. Dyke
Affiliation:
Cross-Infection Reference Laboratory, Colindale Avenue, London, N.W.9, and Department of Microbiology, University of Oxford

Summary

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A comparison was made of the amount of penicillinase produced by strains of Staphylococcus aureus carrying chromosomal penicillinase genes, plasmid penicillinase genes and strains carrying both chromosomal and plasmid genes. Plasmid genes were found to produce at least twice as much enzyme as homologous chromosomal genes. The results suggest that there are, on average, two plasmids per chromosome in exponentially growing cells.

Two inducers were used in these experiments—methicillin and 2-(2′-carboxyphenyl)-benzoyl-6-aminopenicillanicacid (CBAP). The latter proved a more efficient inducer than methicillin and this difference was most apparent when more than one i+ gene was present, which suggests that the efficiency of induction reflects the ability of the inducer to react with represser.

Type
Research Article
Information
Genetics Research , Volume 16 , Issue 1 , August 1970 , pp. 95 - 99
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Asheshov, E. H. (1966). Chromosomal location of the genetic elements controlling penicillinase production in a strain of Staphylococcua aureus. Nature 210, 804–806.CrossRefGoogle Scholar
Asheshov, E. H. (1969). The genetics of penicillinase production in Staphylococcus aureus strain PS 80. Journal of General Microbiology 59, 289–301.CrossRefGoogle Scholar
Asheshov, E. H. & Dyke, K. G. H. (1968). Regulation of the synthesis of penicillinase in diploids of Staphylococcus aureus. Biochemical and Biophysical Research Communications 30, 213–218.CrossRefGoogle ScholarPubMed
Jacob, F. & Monod, J. (1961). Genetic regulatory mechanisms in the synthesis of proteins. Journal of Molecular Biology 3, 318–356.CrossRefGoogle ScholarPubMed
Leggate, J. & Holms, W. H. (1968). Gratuitous synthesis of β-lactamase in Staphylococcua aureus. Journal of Bacteriology 96, 2110–2117.CrossRefGoogle ScholarPubMed
Novick, R. P. (1962). Staphylococcal penicillinase and the new penicillins. Biochemical Journal 83, 229–235.CrossRefGoogle ScholarPubMed
Perret, C. J. (1954). Iodometric assay of penicillinase. Nature, 174, 1012–1013.CrossRefGoogle ScholarPubMed
Poston, S. M. (1966). Cellular location of the genes controlling penicillinase production and resistance to streptomycin and tetracycline in a strain of Staphylococcua aureua. Nature 210, 802–804.CrossRefGoogle Scholar
Richmond, M. H. (1965). Dominance of the inducible state in strains of Staphylococcus aureue containing two distinct penicillinase plasmids. Journal of Bacteriology 90, 370–374.CrossRefGoogle Scholar
Richmond, M. H. (1967). A secondary regulatory region involved in penicillinase synthesis in Staphylococcus aureua. Journal of Molecular Biology 26, 357–360.CrossRefGoogle Scholar
Sweeney, H. M. & Cohen, S. (1968). Wild-type strain of Staphylococcus aureus containing two genetic linkage groups for penicillinase production. Journal of Bacteriology 96, 920–924.CrossRefGoogle ScholarPubMed