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P-element transposase induces male recombination in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Margaret Y. McCarron ,
Atanu Duttaroy ,
Glenn A. Doughty  and
Arthur Chovnick
Margaret Y. McCarron
Affiliation:
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA
Atanu Duttaroy
Affiliation:
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA
Glenn A. Doughty
Affiliation:
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA
Arthur Chovnick*
Affiliation:
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA
*
* Corresponding author.

Summary

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Male recombination in P–M dysgenic crosses has been viewed as a reflection of P-element transposase interacting with P elements. However, recent studies suggest that the transposase may catalyse double-stranded breaks in chromosomal DNA. We have, therefore, introduced P(Δ2–3 ry+) (99B), a single non-mobile P-element transposase source, into the long-standing laboratory true M strains of a flanking lethal crossover selective system, thus facilitating the examination of rare male recombination events as an assay for transposase activity. We find that the rate of male recombination in the presence of this non-mobile P element is greater than twenty times the background rate of male recombination in the control examined prior to introduction of the transposase source.

Type
Research Article
Information
Genetics Research , Volume 54 , Issue 2 , October 1989 , pp. 137 - 142
Copyright
Copyright © Cambridge University Press 1989

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