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The occurrence of long ribosomal transcripts homologous to type I insertions in bobbed mutants of Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Mohamed Makni ,
Mohamed Marrakchi  and
Nicole Prud'Homme
Mohamed Makni*
Affiliation:
Laboratoire de Génétique, Faculté des Sciences, 1060 Tunis, Tunisie
Mohamed Marrakchi
Affiliation:
Laboratoire de Génétique, Faculté des Sciences, 1060 Tunis, Tunisie
Nicole Prud'Homme
Affiliation:
Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvelte, France
*
* Corresponding author.

Summary

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In Drosophila melanogaster up to two thirds of the rDNA genes contain insertion sequences of two types in the 28S coding region. Comparison of the ribosomal insertion transcripts in the wild type and in two bobbed mutants reared at two temperatures showed that the level of type I transcripts is dependent on both the number of genes with type I insertions in the bobbed loci and the intensity of bobbed phenotype. Importantly, a long transcript of 8·7 kb hybridized to the ribosomal probe, the INS I probe and also to the restriction fragment of the rDNA downstream of the point of insertion was found in one bobbed mutant. This result and also those from sandwich hybridization indicate that some interrupted ribosomal genes are functional.

Type
Research Article
Information
Genetics Research , Volume 54 , Issue 2 , October 1989 , pp. 127 - 135
Copyright
Copyright © Cambridge University Press 1989

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