Hostname: page-component-848d4c4894-4rdrl Total loading time: 0 Render date: 2024-06-19T05:03:28.187Z Has data issue: false hasContentIssue false
  • English
  • Français

Non-selfing mutants from selfing (Het−) strains of Physarum polycephalum

Published online by Cambridge University Press:  14 April 2009

N. K. Honey ,
R. T. M. Poulter  and
R. J. Aston
N. K. Honey
Affiliation:
Department of Biochemistry, University of Otago, Dunedin, New Zealand
R. T. M. Poulter
Affiliation:
Department of Biochemistry, University of Otago, Dunedin, New Zealand
R. J. Aston
Affiliation:
Department of Biochemistry, University of Otago, Dunedin, New Zealand

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Plasmodial formation in the Myxomycete Physarum polycephalum is under heterothallic control by a mating type (mt) locus. In natural isolates only amoebae with different mt alleles are able to cross to form diploid plasmodia. A class of mutants isolated from heterothallic amoebae, together with the variant strain CL, is able to form plasmodia in pure clones, designated as selfing. Non-selfing mutants have been isolated from CL and from other selfing amoebae.

This paper reports the isolation and analysis of 64 non-selfing derivatives (designated Npf−) from seven selfing (Het−) strains. The Npf− mutants could be grouped into eight classes on the basis of their crossing and complementation patterns. The possible significance of these mutants is discussed.

Type
Research Article
Information
Genetics Research , Volume 39 , Issue 3 , June 1982 , pp. 261 - 273
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Adler, P. N. & Holt, C. E. (1975). Mating type and the differentiated state in Physarum polycephalum. Developmental Biology 43, 240–253.CrossRefGoogle Scholar
Adler, P. N. & Holt, C. E. (1977). Mutations increasing asexual plasmodium formation in Physarum polycephalum. Genetics 87, 401–420.CrossRefGoogle ScholarPubMed
Anderson, R. W. (1979). Complementation of amoebal-plasmodial transition mutants in Physarum polycephalum. Genetics 91, 409–419.CrossRefGoogle ScholarPubMed
Anderson, R. W. & Dee, J. (1977). Isolation and analysis of amoebal-plasmodial transition mutants in the Myxomycete Physarum polycephalum. Genetical Research 29, 21–34.CrossRefGoogle Scholar
Cooke, D. J. & Dee, J. (1974). Plasmodium formation without change on nuclear DNA content in Physarum polycephalum. Genetical Research 23, 307–317.Google Scholar
Cooke, D. J. & Dee, J. (1975). Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum. Genetical Research 24, 175–187.Google Scholar
Davidow, L. S. & Holt, C. E. (1977). Mutants with decreased differentiation to plasmodia in Physarum polycephalum. Molecular and General Genetics 155, 291–300.CrossRefGoogle Scholar
Dee, J. (1966). Multiple alleles and other factors affecting plasmodium formation in the true slime mould, Physarum polycephalum. Schw. Journal of Protozoology 13, 610–616.CrossRefGoogle Scholar
Dee, J. (1973). Aims and techniques of genetic analysis in Physarum polycephalum. Berichte der deutschen Botanischen Gesellschaft 86, 93–121.CrossRefGoogle Scholar
Dee, J. (1975). Slime moulds in biological research. Science Progress 62, 523–542.Google Scholar
Dee, J. (1978). A gene unlinked to mating-type affecting crossing between strains of Physarum polycephalum. Genetical Research 31, 85–92.Google Scholar
Dee, J. & Poulter, R. T. M. (1970). A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia. Genetical Research 15, 35–41.CrossRefGoogle Scholar
Gorman, J. A., Dove, W. J. & Shaibe, E. (1979). Mutations affecting the initiation of plasmodial development in Physarum polycephalum. Developmental Genetics 1, 47–60.Google Scholar
Gray, W. D. & Alexopoulos, C. J. (1968). Biology of Myxomycetes. New York: Ronald Press.Google Scholar
Honey, N. K., Poulter, R. T. M. & Teale, D. M. (1979). Genetic regulation of differentiation in Physarum polycephalum. Genetical Research 34, 131–142.Google Scholar
Honey, N. K., Poulter, R. T. M. & Winter, P. J. (1981). Selfing mutants from heterothallic strains of Physarum polycephalum. Genetical Research 37, 113–121.Google Scholar
Poulter, R. T. M. (1969). Senescence in the Myxomycete Physarum polycephalum. Ph.D. thesis, University of Leicester.Google Scholar
Poulter, R. T. M. & Dee, J. (1968). Segregation of factors controlling fusion between plasmodia of the true slime mould Physarum polycephalum. Genetical Research 12, 71–79.CrossRefGoogle ScholarPubMed
Poulter, R. T. M. & Honey, N. K. (1977). Genetic analysis of a cross between two homothallic strains of Physarurn polycephalum. Genetical Research 29, 55–63.CrossRefGoogle Scholar
Poulter, R. T. M., Honey, N. K. & Teale, D. M. (1977). The role of the self-sterile mating locus in the repressor-regulated control of differentiation of Physarurn polycephalum. Abstract. Heredity 39, 185.Google Scholar
Shinnick, T. M. & Holt, C. E. (1977). A mutation (gad) linked to mt and affecting asexual plasmodium formation in Physarurn polycephalum. Journal of Bacteriology 131, 247–250.Google Scholar
Wheals, A. E. (1970). A homothallic strain of the Myxomycete Physarurn polycephalum. Genetics 66, 623–633.Google Scholar
Wheals, A. E. (1973). Developmental mutants in a homothallic strain of Physarum polycephalum. Genetical Research 21, 79–86.Google Scholar
Younoman, P. J., Anderson, R. W. & Holt, C. E. (1979). The genetic regulation of zygote formation and zygote differentiation during mating in Physarurn polycephalum. Abstract. Physarum meeting, Laval University, Quebec.Google Scholar