Hostname: page-component-77c89778f8-vpsfw Total loading time: 0 Render date: 2024-07-16T17:19:55.636Z Has data issue: false hasContentIssue false

New compound (1) chromosomes and the production of large quantities of X/O males in Drosophila hydei

Published online by Cambridge University Press:  14 April 2009

H. Beck
Affiliation:
Laboratoire de Génétique animale et végétale, R. de Malagnou 154, Université de Genève, Switzerland

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In view of the exceptional usefulness of Drosophila hydei for the analysis of Y-chromosome activity, a technique has been developed which permits the production of large numbers of males lacking a Y chromosome. It is based on the synthesis of new compound(1) chromosomes which carry sufficient rDNA genes to secure survival in the absence of any Y-chromosomal rDNA. Through non-disjunction in males of suitable stocks, fertile Compound(l) females which lack the Y chromosome, phenotypically distinguishable from their normal sibs, are produced in sufficient number to allow the subsequent breeding of X/O males in gram quantities for biochemical studies.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Baker, W. K. (1971). Evidence for position-effect suppression of the ribosomal RNA cistrons in Drosophila melanogaster. Proceedings of the National Academy of Sciences of the United States of America, Washington 68, 24722476.CrossRefGoogle ScholarPubMed
Beck, H. (1972). The phenotype of X bb+/0 males in Drosophila hydei. Drosophila Information Service 49, 76.Google Scholar
Beck, H. (1973). Some observations on Y-chromosomal bobbed mutants in Drosophila hydei. Drosophila Information Service 50, 9495.Google Scholar
Breugel, F. M. A. van, Ray, A. & Gloor, H. (1968). A comparison of banding patterns in salivary gland chromosomes of two species of Drosophila. Genetica 39, 166192.Google ScholarPubMed
Breugel, F. M. A. van (1970). An analysis of white-mottled mutants in Drosophila hydei, with observations on XY exchanges in the male. Genetica 41, 589625.CrossRefGoogle ScholarPubMed
Frei, H. (1974). Unterschiedliche Strahlenempfindlichkeit in der Spermatogenese von Drosophila hydei, Letalraten und die Bedeutung der arteigenen genetischen Organisation. Archiv für Genetik 47, 136171.Google Scholar
Gregg, T. G. (1957). The production of attached-X chromosomes in Drosophila hydei. University of Texas Publication, no. 5721, pp. 238245.Google Scholar
Hennig, W. (1968). Ribonucleic acid synthesis of the Y-chromosome of Drosophila hydei. Journal of Molecular Biology 38, 227239.Google Scholar
Hess, O. & Meyer, G. F. (1968). Genetic activities of the Y chromosome in Drosophila during spermatogenesis. Advances in Genetics 14, 171223.CrossRefGoogle Scholar
Lindsley, D. L. & Grell, E. H. (1968). The Genetic Variations of Drosophila melanogaster. Carnegie Institution of Washington Publication, no. 627.Google Scholar
Meyer, G. F. (1963). Die Funktionsstrukturen des Y-chromosoms in den Spermatocytenkernen von Drosophila hydei, D. neohydei, D. repleta und einigen anderen Drosophila-Arten. Chromosoma (Berlin) 14, 207255.CrossRefGoogle Scholar