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Modification of the suppressor phenotype of thymine requiring strains of Escherichia coli

Published online by Cambridge University Press:  14 April 2009

Muriel B. Herrington ,
Johnny Basso ,
Maria Faraci  and
Chantal Autexier
Muriel B. Herrington*
Affiliation:
Biology Department, Concordia University, 1455 de Maisonneuve Blvd. W., Montreal, Quebec, Canada H3G 1M8
Johnny Basso
Affiliation:
Biology Department, Concordia University, 1455 de Maisonneuve Blvd. W., Montreal, Quebec, Canada H3G 1M8
Maria Faraci
Affiliation:
Department of Microbiology and Immunology, McGill University, 3775 University, Montreal, Quebec, Canada, H3A 2B4
Chantal Autexier
Affiliation:
Woman's College Hospital, 76 Glenville St, Toronto, Ontario, Canada, M5S 1B2
*
*Corresponding author.

Summary

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Thymine requiring strains of Escherichia coli suppress nonsense and frameshift mutations during translation. Strains with different genetic backgrounds exhibited different nonsense suppression spectra and showed differences in the apparent suppression efficiency. Part of this strain difference is due to a presumably novel gene (tsmA) mapping near 39 min. This gene affects the spectrum and apparent efficiency of suppression, and appears to affect the utilization of thymidine.

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 3 , December 1991 , pp. 185 - 192
Copyright
Copyright © Cambridge University Press 1991

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