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A method for isolating paralysed (mot−) mutants from non-flagellated cells of Salmonella

Published online by Cambridge University Press:  14 April 2009

Masatoshi Enomoto
Masatoshi Enomoto
Affiliation:
National Institute of Genetics, Mishima, Shizuoka-ken, Japan

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A method for concentrating flagella-paralysed (mot−) cells existing in a large number of non-flagellated cells of Salmonella was invented. The mot− cells expressing the phase-1 flagellar antigen i were agglutinated by anti-i serum with carriers, the dead cells of TM2 expressing the phase-1 antigen i; only the agglutinated cells were harvested by a low speed of centrifugation and incubated in broth. About tenfold concentration was brought about by one treatment, which was repeated for further concentration. By this method, the motA257 motC244 double mutant which was produced by transduction at a frequency of about 3 × 10−6 per non-flagellated recipient cell was isolated from the broth culture concentrated five times, in which about 30% of the cells were the double mutant.

Type
Short Papers
Information
Genetics Research , Volume 14 , Issue 1 , August 1969 , pp. 89 - 92
Copyright
Copyright © Cambridge University Press 1969

References

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