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Meiosis in Schizophyllum commune: premeiotic DNA replication and meiotic synchrony induced with hydroxyurea

Published online by Cambridge University Press:  14 April 2009

Pheya Carmi ,
Y. Koltin  and
Judith Stamberg
Pheya Carmi
Affiliation:
Microbiology Department, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel
Y. Koltin
Affiliation:
Microbiology Department, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel
Judith Stamberg
Affiliation:
Microbiology Department, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel

Summary

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Hydroxyurea (HU) effectively inhibits meiosis in Schizophyllum. The predominant cytological stage in inhibited fruit bodies is fusion. The inhibition is reversible and makes possible synchronization of a naturally nonsynchronous system. Microphotometric determinations of the DNA content in prefusion nuclei treated with HU suggest that premeiotic DNA replication occurs in prefusion nuclei. Synaptinemal complexes are not completed in HU-treated nuclei, suggesting that this event is dependent on premeiotic DNA replication.

Type
Research Article
Information
Genetics Research , Volume 31 , Issue 3 , June 1978 , pp. 215 - 226
Copyright
Copyright © Cambridge University Press 1978

References

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