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Mapping of purine markers in Escherichia coli K 12

Published online by Cambridge University Press:  14 April 2009

A. H. Stouthamer ,
P. G. de Haan  and
H. J. J. Nijkamp
A. H. Stouthamer
Affiliation:
Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands
P. G. de Haan
Affiliation:
Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands
H. J. J. Nijkamp
Affiliation:
Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands

Extract

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A number of mutants of E. coli K 12, deficient in purine biosynthesis, have been isolated and the biochemical blocks have been determined. The mutations were mapped in conjugation experiments. In some cases the differences in penetration times were too small to determine the exact order of the loci by interrupted mating experiments. In these cases the recombination frequencies were determined in four factor crosses. In this way the location of eleven different purine markers has been determined. The loci are scattered over the chromosome. Only two groups of linked genes were found.

Type
Research Article
Information
Genetics Research , Volume 6 , Issue 3 , November 1965 , pp. 442 - 453
Copyright
Copyright © Cambridge University Press 1965

References

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