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Isolation and characterization of bleomycin-resistant clones of CHO cells

Published online by Cambridge University Press:  14 April 2009

Sylvia Brabbs  and
J. R. Warr
Sylvia Brabbs
Affiliation:
Biology Department, University of York, Heslington, York, YO1 5DD, U.K.
J. R. Warr
Affiliation:
Biology Department, University of York, Heslington, York, YO1 5DD, U.K.

Summary

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Five clones of Chinese hamster ovary cells with increased resistance to bleomycin have been isolated following ethylmethanesulphonate mutagenesis. Resistance was stable in three of the clones, but unstable in the other two. One of the stably resistant clones was cross resistant to unrelated drugs, and in contrast to the parental cells, its response to bleomycin was potentiated by tween 80. These two observations suggested a membrane alteration in the resistant clone. There was no significant difference in bleomycin-inactivating enzyme activity between the parental and resistant clones.

Type
Research Article
Information
Genetics Research , Volume 34 , Issue 3 , December 1979 , pp. 269 - 279
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

Blum, R. H., Carter, S. K. & Agre, K. (1973). A clinical review of bleomycin – a new antineoplastic agent. Cancer, 31, 903–914.3.0.CO;2-N>CrossRefGoogle ScholarPubMed
Crooke, S. T. & Brather, W. T. (1976). Bleomycin, a review. Journal of Medicine 7, 333–428.Google ScholarPubMed
Demars, R. (1974). Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detection. Mutation Research 24, 335–364.CrossRefGoogle ScholarPubMed
Hittleman, W. N. & Rao, P. N. (1974). Bleomycin induced damage in prematurely condensed chromosomes and its relationship to cell cycle progression in CHO cells. Cancer Research 34, 3433–3439.Google Scholar
Kao, F. T. & Puck, T. T. (1968). Genetics of somatic mammalian cells. VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proceedings of the National Academy of Sciences. U.S. 60, 1275–1281.CrossRefGoogle ScholarPubMed
Kao, F. T. & Puck, T. T. (1970). Genetics of somatic mammalian cells; linkage studies in human – Chinese hamster hybrids. Nature Lond. 228, 329–332.CrossRefGoogle Scholar
Ling, V. (1975). Drug resistance and membrane alteration in mutants of mammalian cells. Canadian Journal of Genetics and Cytology 17, 503–515.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Mayaki, M., Ono, T., Hori, S. & Umezawa, H. (1975). Binding of bleomycin to DNA in bleomycin sensitive and resistant rat ascites hepatoma cells. Cancer Research 351, 2015–2019.Google Scholar
Muller, W. E. G., Totsuka, A., Nosser, I., Zahn, R. K. & Umezawa, H. (1975). Bleomycin inhibition of DNA synthesis in isolated enzyme systems and in intact cells. Biochemical Pharmacology 24, 911–915.CrossRefGoogle Scholar
Muller, W. E. G., Yamazaki, Z., Breter, M. & Zahn, R. K. (1972). Action of bleomycin on DNA and RNA. European Journal of Biochemistry 31, 518–525.CrossRefGoogle ScholarPubMed
Muller, W. E. G. & Zahn, R. K. (1977). Bleomycin, an antibiotic that removes thymine from double stranded DNA. Progress in Nucleic Acid Research and Molecular Biology 20, 21–57.Google Scholar
Nagatsu, M., Richart, R. M. & Lambert, A. (1972). Effects of bleomycin on the cell cycle of Ehrlich ascites carcinoma. Cancer Research 32, 1966–1970.Google ScholarPubMed
Ohnuma, T., Holland, J. S.Masuda, H. (Waleguda, J. A. & Goldberg, J. A. (1974). Microbiological assay of bleomycin; inactivation, tissue distribution and clearance. Cancer 33, 1230–1238.3.0.CO;2-C>CrossRefGoogle Scholar
Riehm, H. & Biedler, J. L. (1972). Potentiation of drug effect by tween 80 in Chinese hamster cells resistant to actinomycin D and daunomycin. Cancer Research 32, 1195–1200.Google ScholarPubMed
Paika, K. O. & Krishnan, A. (1973). Bleomycin induced chromosomal aberrations in cultured mammalian cells. Cancer Research 33, 961–965.Google ScholarPubMed
Satoh, H. & Shinura, H. (1969). Effect of bleomycin on ascites hepatoma in rat. Progress in Medicine (Tokyo), 69, 669–672.Google Scholar
Saunders, P. P. & Schultz, G. A. (1972). Mechanism of action of bleomycin. I. Bacterial growth studies. Biochemical Pharmacology 21, 1657–1666.CrossRefGoogle ScholarPubMed
Spangler, M., Sartiano, G. P., Coetzee, M. L. & Ove, P. (1976). Effects of bleomycin on 3H thymidine 5 triphosphate into host liver and hepatoma nuclei. Cancer Research 36, 1339–1346.Google ScholarPubMed
Terzi, M. (1974). Chromosomal variation and the origin of drug resistant mutants in mammalian cell lines. Proceedings of the National Academy of Science, U.S. 71, 5027–5031.CrossRefGoogle ScholarPubMed
Tobey, R. A. (1972). Arrest of Chinese hamster cells in G2 following treatment with the antitumour drug bleomycin. Journal of Cell Physiology, 79, 259–266.CrossRefGoogle Scholar
Umezawa, H. (1971). Natural and artificial bleomycins; chemistry and anti-tumour activity. Pure and Applied Chemistry 28, 665–680.CrossRefGoogle Scholar
Wanatabe, M., Takabe, Y., Katsumata, T. & Terasima, T. (1974). Effects of bleomycin on progression through the cell cycle of mouse L cells. Cancer Research, 34, 2726–2732.Google Scholar