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Inheritance of a meiotic abnormality that causes the ovulation of primary oocytes and the production of digynic triploid mice

Published online by Cambridge University Press:  14 April 2009

John D. West ,
Sheila Webb  and
Matthew H. Kaufman
John D. West*
Affiliation:
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, UK
Sheila Webb
Affiliation:
Department of Anatomy, Medical School, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK
Matthew H. Kaufman
Affiliation:
Department of Anatomy, Medical School, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK
*
* Corresponding author.
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Previous studies have demonstrated that the LT/SvKau strain of mice ovulates a high proportion of oocytes as diploid primary oocytes rather than secondary oocytes. These ovulated primary oocytes are arrested at meiotic metaphase I but may be fertilized to produce digynic triploid embryos. In the present study, 40·4% of eggs analysed from LT/SvKau females were ovulated as primary oocytes, compared to 1·2% from control C57BL/Ws strain mothers. These two inbred strains were intercrossed to produce eight sets of Fl, F2 and backcross females and the frequency of triploidy was investigated. The results are compatible with segregation of a co-dominant, autosomal gene that has a major influence on the incidence of triploidy. We suggest that the provisional gene symbol Poo (primary oocyte ovulation) be assigned to this gene, with alleles Pool (the ‘mutant’ allele present in the LT/SvKau strain) and Poob (the normal allele present in C57BL/Ws mice). Poo is incompletely penetrant and has variable expressivity because the proportion of oocytes ovulated as primary oocytes by LT/SvKau mice was variable and, in some cases, nil. In putative Pool/Poob heterozygotes the frequency of ovulated primary oocytes was increased only marginally (from 1·2% to 66%) by the presence of one copy of the Pool allele, but this increase was found consistently (in two reciprocal Fl crosses) and was statistically significant. No evidence was found for tight genetic linkage between Poo and two Mendelian loci (brown on chromosome 4 and glucose phosphate isomerase on chromosome 7), that were segregating in the crosses. The Pool mutant in the LT/SvKau strain of mice provides a valuable resource to study the cell and molecular biology of mammalian oocyte maturation and the control of meiosis.

Type
Research Article
Information
Genetics Research , Volume 62 , Issue 3 , December 1993 , pp. 183 - 193
Copyright
Copyright © Cambridge University Press 1993

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