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Infectious transfer of a fertility factor in Streptomyces coelicolor

Published online by Cambridge University Press:  14 April 2009

A. Maria Puglia ,
Isabella Spada-Sermonti ,
Santina Basile ,
Fara Misuraca  and
G. Sermonti
A. Maria Puglia
Affiliation:
Istituto di Genetica, Università di Palermo, Italy
Isabella Spada-Sermonti
Affiliation:
Istituto di Genetica, Università di Palermo, Italy
Santina Basile
Affiliation:
Istituto di Genetica, Università di Palermo, Italy
Fara Misuraca
Affiliation:
Istituto di Genetica, Università di Palermo, Italy
G. Sermonti
Affiliation:
Istituto di Genetica, Università di Palermo, Italy

Summary

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Initial Fertility (IF) strains of Streptomyces coelicolor are able to convert recipient strains (UF) to the IF condition by contact, without concomitant transfer of chromosomal markers. The conversion is prevented by the presence of acridine orange in the medium of the mixed culture. Acridine orange is also moderately effective in inducing the formation of UF variants from IF-treated strains. No effect of the drug is observed on UF variant formation from Normal Fertility (NF) strains nor on the behaviour of the fertility factor in NF × UF mixed cultures. The hypothesis is put forward that the fertility factor works as an episome in S. coelicolor, fixed to the chromosome in the NF strains, free in the IF strains and missing in the UF strains.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 2 , April 1973 , pp. 107 - 113
Copyright
Copyright © Cambridge University Press 1973

References

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