Hostname: page-component-848d4c4894-8bljj Total loading time: 0 Render date: 2024-06-16T19:15:07.402Z Has data issue: false hasContentIssue false
  • English
  • Français

The induction of ouabain-resistant mutants by N-methyl-N′-nitro-N-nitrosoguanidine in Chinese hamster cells

Published online by Cambridge University Press:  14 April 2009

Peter J. Davies  and
Jim Parry
Peter J. Davies
Affiliation:
Department of Genetics, University College of Swansea, Swansea SA2 8PP, U.K.
Jim Parry
Affiliation:
Department of Genetics, University College of Swansea, Swansea SA2 8PP, U.K.

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Clones of V79 Chinese hamster cells resistant to the steroid compound ouabain have been induced by mutagen treatment with MNNG (= N-methyl-N′-nitro-N-nitrosoguanidine). The frequency of resistant colonies was increased from spontaneous value of 2 × 10−6 to a frequency of 1·2 × 10−3 with MNNG treatment.

The highest number of induced mutants appeared when an expression time pf 40 h was allowed between MNNG treatment and addition of ouabain.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 3 , December 1974 , pp. 311 - 314
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Arlett, C. F. (1972). Mutation testing with cultured mammalian cells. Laboratory Practice 21, 420–423.Google ScholarPubMed
Baker, R. M., Brunette, D. M., Mankovitz, R., Thompson, L. H., Whitmore, G. F., Simmovitch, L. & Till, J. E. (1974). Ouabain resistant mutants of mouse and hamster cells in culture. Cell 1, 9–21.CrossRefGoogle Scholar
Bridges, B. A. & Huckle, J. (1970). Mutagenesis of cultured mammalian cells by X-irradiation and UV light. Mutation Research 10, 141–151.CrossRefGoogle Scholar
Chu, E. H. Y. & Mailing, H. V. (1968). Mammalian cell genetics. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proceedings of the National Academy of Science, U.S.A. 61, 1306–1312.CrossRefGoogle ScholarPubMed
Duncan, M. E. & Brookes, P. (1973). The induction of aza-guanine resistant mutants in cultured Chinese hamster cells by reactive derivatives of carcinogenic hydrocarbons. Mutation Research 21, 107–118.CrossRefGoogle Scholar
Mayhew, E. & Lewinson, C. (1968). Reversibility of ouabain induced inhibition of cell division and cation transport in Erlich ascites cells. Journal of Cellular Physiology 72, 73–78.CrossRefGoogle Scholar
McDonald, T. F., Sachs, H. G., Orr, C. W. M. & Ebert, J. D. (1972). Multiple effects of ouabain on BHK cells. Experimental Cell Research 74, 201–206.CrossRefGoogle ScholarPubMed
Wheeler, K. P. & Whittam, R. (1962). Some properties of kidney adenosine triphosphatase relevant to active cation transport. Biochemical Journal 85, 495–507.CrossRefGoogle ScholarPubMed