Hostname: page-component-77c89778f8-7drxs Total loading time: 0 Render date: 2024-07-17T04:59:45.739Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetics and biochemistry of 5-Bromodeoxyuridine resistance in Physarum polycephalum

Published online by Cambridge University Press:  14 April 2009

Asgeir Lunn ,
David Cooke  and
Finn Haugli
Asgeir Lunn
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway
David Cooke
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway
Finn Haugli
Affiliation:
Institute of Medical Biology, University of Tromsø, 9000 Tromsø, Norway

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

5-Bromodeoxyuridine (BrdU)-resistant mutants of Physarum polycephalum were isolated as colonies of myxamoebae growing on BrdU-substituted bacteria after exposure to long-wave ultraviolet light (UV). Twenty-four such mutants were studied. They all show Mendelian segregation in crosses with wild type. Plasmodia constructed from mutant amoebae were all deficient in deoxythymidine incorporation. Extracts made from selected plasmodia showed that all except one had low thymidine kinase activity.

Genetical and biochemical complementation studies revealed two complementation groups: 23 mutants, bur A, had low thymidine kinase while 1 mutant, bur B, had normal thymidine kinase levels.

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 1 , August 1977 , pp. 1 - 12
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Anderson, R., Cooke, D. J. & Dee, J. (1976). Apogamic development of plasmodia in Physarum polycephalum: a cinematographic analysis. Protoplasma 89. 29–40.CrossRefGoogle ScholarPubMed
Bright, S. W. J. & Northcote, D. H. (1974). Protoplast regeneration from normal and bromodeoxyuridine-resistant Sycamore callus. Journal of Cell Science 16. 445–463.CrossRefGoogle Scholar
Carl, P. L. (1970). Escherichia coli mutants with temperature-sensitive synthesis of DNA. Molecular and General Genetics 109. 107–122.CrossRefGoogle ScholarPubMed
Chace, K. V. & Hall, D. H. (1973). Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity. Journal of Virology 12. 343–348.CrossRefGoogle ScholarPubMed
Cooke, D. J. (1974). Studies on the Colonia isolate of Physarum polycephalum. Ph.D. thesis, University of Leicester.Google Scholar
Cooke, D. J. & Dee, J. (1974). Plasmodium formation without change in nuclear DNA content in Physarum polycephalum. Genetical Research 23. 307–317.CrossRefGoogle ScholarPubMed
Cooke, D. J. & Dee, J. (1975). Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum. Genetical Research 24. 175–187.CrossRefGoogle Scholar
Daniel, J. W. & Baldwin, H. H. (1969). Methods of culture for plasmodial myxomycetes. In Methods in Cell Physiology 1, 9–41.CrossRefGoogle Scholar
Dee, J. (1966). Genetic analysis of actidione-resistant mutants in the myxomycete Physarum polycephalum. Genetical Research 8. 101–110.CrossRefGoogle ScholarPubMed
Dee, J. & Poulter, R. T. M. (1970). A gene conferring actidione resistance and abnormal morphology on Physarum plasmodia. Genetical Research 15. 35–41.CrossRefGoogle Scholar
Freed, J. J. & Mezger-Freed, L. (1973). Origin of thymidine kinase deficient (TK−) haploid frog cells via an intermediate thymidine transport deficient (TT−) phenotype. Journal of Cell Physiology 82. 199–212.Google ScholarPubMed
Furlong, N. B. (1963). A rapid assay for nucleotide kinases using 14[C]- or 3[H]-labelled nucleotides. Analytical Chemistry 5. 515–522.Google Scholar
Gröbner, P., Finkenstedt, G., Woertz, G., Wolf, H. & Sachsenmater, W. (1975). Enzyme variants of thymidine kinase in Physarum polycephalum: Changing pattern during the synchronous mitotic cycle. Hoppe Seyler's Zeitschrift für Physiologisches Chemie 356, 235.Google Scholar
Haugli, F. B. (1971). Mutagenesis, selection and genetic analysis in Physarum polycephalum. Ph.D. thesis, University of Wisconsin.Google Scholar
Haugli, F. B. & Dove, W. F. (1972). Mutagenesis and mutant selection in Physarum polycephalum. Molecular and General Genetics 118. 109–124.CrossRefGoogle ScholarPubMed
Hildebrandt, A. & Sauer, H. (1973). Thymidine phosphorylation in the cell cycle of Physarum polycephalum and the effect of 5-fluoro-2-deoxyuridine and hydroxyurea. Biochimica et Biophysica Acta 294. 8–14.CrossRefGoogle Scholar
Hiraga, S., Igarashi, K. & Yura, T. (1967). A deoxy-thymidine kinase-dencient mutant of Escherichia coli. Biochimica et Biophysica Acta 145. 41–51.CrossRefGoogle Scholar
Kao, F. T. & Puck, T. T. (1969). Genetics of somatic mammalian cells. VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proceedings of the National Academy of Sciences, U.S.A. 60. 1275–1281.CrossRefGoogle Scholar
Kit, S., Leung, W. C. & Kaplan, L. A. (1973). Discrete molecular forms of thymidine kinase in mitochondria of normal and 5-bromodeoxyuridine resistant HeLa cells. European Journal of Biochemistry 39. 43–48.CrossRefGoogle Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193. 265–275.CrossRefGoogle ScholarPubMed
Ohyama, K. (1974). Properties of 5-bromodeoxyuridine resistant lines of higher plant cells in liquid culture. Experimental Cell Research 89. 31–38.CrossRefGoogle ScholarPubMed
Roufa, D. J., Sadow, B. N. & Caskey, C. T. (1973). Derivation of TH− clones from revertant TK+ mammalian cells. Genetics 75. 515–530.CrossRefGoogle Scholar
Sachsenmaier, W. & Ives, D. H. (1965). Periodische Anderungen der thymidin kinase Aktivitat in Synchronen Mitose-cyclus von Physarum polycephalum. Biochemische Zeitschrift 343. 399–406.Google ScholarPubMed
Sachsenmaier, W., Fournier, D. V. & Gürtler, K. F. (1967). Periodic thymidine kinase production in synchronous plasmodia of Physarum polycephalum; inhibition by actinomycin and actidione. Biochemical and Biophysical Research Communications 27, 655–660.CrossRefGoogle Scholar
Taylor, A. T., Stafford, M. A. & Jones, O. W. (1972). Properties of thymidine kinase partially purified from human fetal and adult tissue. Journal of Biological Chemistry 247. 1930–1935.CrossRefGoogle ScholarPubMed
Wheals, A. E. (1970). A homothallic strain of the Myxomycete Physarum polycephalum. Genetics 66. 623–633.CrossRefGoogle ScholarPubMed