Hostname: page-component-76fb5796d-wq484 Total loading time: 0 Render date: 2024-04-26T02:33:02.140Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic analysis of a large autosomal region in Caenorhabditis elegans by the use of a free duplication

Published online by Cambridge University Press:  14 April 2009

A. M. Howell ,
S. G. Gilmour ,
R. A. Mancebo  and
A. M. Rose
A. M. Howell
Affiliation:
Department of Medical Genetics, University of British Columbia, Vancouver, Canada V6T 1W5
S. G. Gilmour
Affiliation:
Department of Medical Genetics, University of British Columbia, Vancouver, Canada V6T 1W5
R. A. Mancebo
Affiliation:
Department of Medical Genetics, University of British Columbia, Vancouver, Canada V6T 1W5
A. M. Rose
Affiliation:
Department of Medical Genetics, University of British Columbia, Vancouver, Canada V6T 1W5
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In this paper we describe the use of a free duplication, sDp2 (I;f), for the recovery, maintenance, and analysis of mutations defining essential genes in the left third of Linkage Group I of Caenorhabditis elegans. The lethals were induced in a strain of genotype (sDp2) + /dpy-5 + unc-13/ dpy-5 unc-15 +, using either 12 mM ethylmethane sulphonate or 1500 r of gamma radiation. Lethal mutations linked to the dpy-5 unc-13 chromosome were recognized by the absence of Dpy-5 Unc-13 individuals amongst the self progeny and were maintained by isolating Unc-13 hermaphrodites. These strains – which have two mutant alleles of the essential gene and a wild-type allele on the duplication – are balanced, since crossing-over does not occur between sDp2 and the normal homologues. Using this sytem we have recovered 58 EMS-induced mutations. These have been characterized with regard to map position and complementation. Twenty-nine of the EMS-induced mutations lie to the left of dpy-5 and define 20 complementation groups; 3 were inseparable from dpy-5 and define 3 complementation groups; 21 were to the right and define 17 complementation groups. Among a set of 29 gamma radiation-induced lethal mutations, 17 appear to be single gene mutations or are very small deletions. We estimate that we have identified from one-sixth to one-half of the essential genes in the sDp2 region.

Type
Research Article
Information
Genetics Research , Volume 49 , Issue 3 , June 1987 , pp. 207 - 213
Copyright
Copyright © Cambridge University Press 1987

References

Anderson, P. & Brenner, S. (1984). A selection for myosin heavy chain mutants in the nematode Caenorhabditis elegans. Proceedings of the National Academy of Sciences, USA 81, 4470–4474.CrossRefGoogle ScholarPubMed
Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71–94.CrossRefGoogle ScholarPubMed
Fodor, A. & Deak, P. (1985). The isolation and genetic analysis of a C. elegans translocation (szTl) strain bearing an X-chromosome balancer. Journal of Genetics 64, 143–157.CrossRefGoogle Scholar
Hodgkin, J., Horvitz, H. R. & Brenner, S. (1979). Nondisjunction mutants of the nematode Caenorhabditis elegans. Genetics 91, 67–94.CrossRefGoogle ScholarPubMed
Judd, B. H., Shen, M. W. & Kaufman, T. C. (1972). The anatomy and function of a segment of the X-chromosome of Drosophila melanogaster. Genetics 71, 139–156.CrossRefGoogle ScholarPubMed
Meneely, P. M. & Herman, R. K. (1979). Lethals, steriles, and deficiencies in a region of the X-chromosome of Caenorhabditis elegans. Genetics 92, 99–115.CrossRefGoogle Scholar
Rogalski, T. R. & Baillie, D. L. (1985). Genetic organization of the unc-22 IV region of Caenorhabditis elegans. Molecular and General Genetics 201, 409–414.CrossRefGoogle ScholarPubMed
Rogalski, T. M., Moerman, D. G. & Baillie, D. L. (1982). Essential genes and deficiencies in the unc-22 region of Caenorhabditis elegans. Genetics 102, 725–736.CrossRefGoogle ScholarPubMed
Rose, A. M. (1980). Genetic studies on the gene coding for paramyosin in Caenorhabditis elegans: unc-15 and the adjacent region. Ph.D. thesis, Simon Fraser University, Burnaby, B.C., Canada.Google Scholar
Rose, A. M. & Baillie, D. L. (1979). The effect of temperature and parental age on recombination and nondisjunction in Caenorhabditis elegans. Genetics 92, 21–30.CrossRefGoogle ScholarPubMed
Rose, A. M. & Baillie, D. L. (1980). Genetic organization of the region around unc-15 (I), a gene affecting paramyosin in Caenorhabditis elegans. Genetics 96, 639–648.CrossRefGoogle Scholar
Rose, A. M., Baillie, D. L. & Curran, J. (1984). Meiotic pairing behavior of two free duplications of linkage group I in Caenorhabditis elegans. Molecular and General Genetics 195, 52–56.CrossRefGoogle Scholar
Rosenbluth, R. E., Cuddeford, C. & Baillie, D. L. (1983). Mutagenesis in Caenorhabditis elegans. I. A rapid eukaryotic mutagen test system using the reciprocal translocation, eT1(III;V). Mutation Research 110, 39–48.CrossRefGoogle Scholar
Rosenbluth, R. E. & Baillie, D. L. (1981). Analysis of a reciprocal translocation, eT1(III;V), in Caenorhabditis elegans. Genetics 99, 415–428.CrossRefGoogle ScholarPubMed
Sigurdson, D. C., Spanier, G. J. & Herman, R. K. (1984). C. elegans deficiency mapping. Genetics 108, 331–345.CrossRefGoogle ScholarPubMed
Waterston, R. K., Fishpool, R. M. & Brenner, S. (1977). Mutants affecting paramyosin in Caenorhabditis elegans. Journal of Molecular Biology 117, 679–698.CrossRefGoogle ScholarPubMed