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Fertility of sperm from t/ + mice: evidence that + − bearing sperm are dysfunctional

Published online by Cambridge University Press:  14 April 2009

Patricia Olds-Clarke
Affiliation:
Department of Anatomy, Temple University School of Medicine, Philadelphia, PA 19140
Betsy Peitz
Affiliation:
Department of Biology, California State University at Los Angeles, Los Angeles, CA 90032
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Male mice carrying one complete t haplotype transmit it to virtually all offspring. This implies that either the sperm carrying the t haplotype have an advantage in fertilization, or that the sperm not carrying a t haplotype are dysfunctional. To distinguish between these possibilities, epididymal sperm from tw32/ + and congenic + / + males were artificially inseminated into the uterus of T / + females, and the transmission ratio determined from the phenotype of the offspring. After artificial insemination (AI) of sperm from tw32/ + males, the mean ratio was 0·95 (3 experiments, 5 litters), demonstrating that the transmission ratio remains high after AI into the uterus. After AI of a mixture of equal numbers of motile sperm from tw32/ + and congenic + / + males, the mean ratio was 0·22 (6 experiments, 8 litters). These results suggest that sperm carrying a t haplotype from t/ + males are not superior to sperm from + / + males, and therefore imply that sperm not carrying a t haplotype from t/ + males are dysfunctional.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1986

References

Bennett, D. (1975). The T-locus of the mouse. Cell 6, 441454.CrossRefGoogle Scholar
Braden, A. W. H. (1972). T-locus in mice: segregation distortion and sterility in the male. In Proceedings of the International Symposium: The Genetics of the Spermatozoon (ed. Beatty, R. A. and S., Gluecksohn-Waelsch), pp. 289305. Denmark: Bogtrykkeriet Forum.Google Scholar
Braden, A. W. H. and Gluecksohn-Waelsch, S. (1958). Further studies on the effect of the T locus in the house mouse on male fertility. Journal of Experimental Zoology 138, 431452.CrossRefGoogle Scholar
Buehr, M. & McLaren, A. (1984). Interlitter variation in progeny of chimaeric male mice. Journal of Reproduction and Fertility 72, 213221.CrossRefGoogle ScholarPubMed
Edwards, R. G. (1955). Selective fertilization following the use of sperm mixtures in the mouse. Nature 175, 215216.CrossRefGoogle ScholarPubMed
Hammerberg, C. & Klein, J. (1975). Linkage disequilibrium between H-2 and t complexes in chromosome 17 of the mouse. Nature 258, 296299.CrossRefGoogle Scholar
McGrath, J. & Hillman, N. (1980). The in vitro transmission frequency of the t 12 mutation in the mouse. Journal of Embryology and experimental Morphology 60, 141151.Google ScholarPubMed
Mickova, M. & Ivanyi, P. (1974). Sex-dependent and H-2 linked influence on expressivity of the brachyury gene in mice. Journal of Heredity 65, 369372.CrossRefGoogle ScholarPubMed
Nadijcka, M. & Hillman, N. (1980). Differences in cAMP levels in tn- and non-tn-bearing mouse spermatozoa. Biology of Reproduction 11, 11021105.CrossRefGoogle Scholar
Olds-Clarke, P. & Becker, A. (1978). The effect of the T locus on sperm penetration in vivo in the house mouse. Biology of Reproduction 18, 132140.CrossRefGoogle Scholar
Seitz, A. W. & Bennett, D. (1975). Transmission distortion of t-haplotypes is due to interactions between meiotic partners. Nature 313, 143144.CrossRefGoogle Scholar
Silver, L. M. (1986). Mouse t haplotypes. Ann. Rev. Genet. 19, 179208.CrossRefGoogle Scholar
Silver, L. M. & Olds-Clarke, P. (1984). Transmission ratio distortion of mouse t haplotypes is not a consequence of wild-type sperm degeneration. Developmental Biology 105, 250252.CrossRefGoogle Scholar
Tessler, S., Carey, J. E. & Olds-Clarke, P. (1981). Mouse sperm motility affected by factors in the T/t complex. Journal of Experimental Zoology 217, 277285.CrossRefGoogle ScholarPubMed
Tokuyasu, K. T., Peacock, W. J. & Hardy, R. W. (1977). Dynamics of spermiogenesis in Drosophila melanogaster. VII. effects of Segregation Distorter (SD) chromosome. Journal of Ultrastructure Research 58, 96107.CrossRefGoogle ScholarPubMed
West, J. D., Frels, W. I., Papaioannou, V. E., Karr, J. P. & Chapman, V. M. (1977). Development of interspecific hybrids of Mus. Journal of Embryology and experimental Morphology 41, 233243.Google ScholarPubMed
Wolf, D. & Inoue, M. (1976). Sperm concentration dependency in the fertilization and the zonae sperm binding properties of mouse eggs inseminated in vitro. Journal of Experimental Zoology 196, 2738.CrossRefGoogle ScholarPubMed
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