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Estimation of meiosis and sporulation efficiencies in the fission yeast by ascus analysis*

Published online by Cambridge University Press:  14 April 2009

G. B. Calleja ,
M. Zuker  and
Byron F. Johnson
G. B. Calleja
Affiliation:
Division of Biological Sciences, National Research Council of Canada, Ottawa K1A 0R6, Canada
M. Zuker
Affiliation:
Division of Biological Sciences, National Research Council of Canada, Ottawa K1A 0R6, Canada
Byron F. Johnson
Affiliation:
Division of Biological Sciences, National Research Council of Canada, Ottawa K1A 0R6, Canada

Summary

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Populations of linear asci are classified according to the number of spores in an ascus. The resultant five numerical classes are further classified into ten spatial classes according to the arrangement of the spores in an ascus and, by inference, into ten historical classes according to the number and origins of failures during the developmental process. An analysis of the observed frequencies of numerical classes allows derivation of the efficiencies of the first meiotic division, the second meiotic division and sporulation in a fission yeast. The analytical method may be useful in locating the site of action of sporulation inhibitors and in identifying meiosis mutants from sporulation mutants.

Type
Research Article
Information
Genetics Research , Volume 33 , Issue 2 , April 1979 , pp. 109 - 119
Copyright
Copyright © Cambridge University Press 1979

References

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