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Effect of inhibition of DNA synthesis on u.v. sensitive Bs strains of Escherichia coli

Published online by Cambridge University Press:  14 April 2009

Michael H. L. Green ,
John Donch ,
Young S. Chung  and
Joseph Greenberg
Michael H. L. Green
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California 94301, U.S.A.
John Donch
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California 94301, U.S.A.
Young S. Chung
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California 94301, U.S.A.
Joseph Greenberg
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California 94301, U.S.A.

Extract

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The effect of nalidixic acid, a specific inhibitor of DNA synthesis, on Escherichia coli strain B (lon) and its u.v.-sensitive derivatives is examined. Strain B itself is sensitive to nalidixic acid, whereas its u.v.-resistant derivative B/r is resistant.

It is shown that in all exr A strains, in which u.v.-induced filamentation is suppressed, resistance to nalidixic acid is increased. Among exr A strains, Bs4 is exceptionally resistant to nalidixic acid. This is because nalidixic acid kills only growing cells and strain Bs4, a try auxotroph, may grow poorly under the conditions used to test nalidixic acid.

The uvr genes of the HCR strains Bs1, Bs8 and Bs12 do not suppress u.v.-induced filamentation nor do they affect the response to nalidixic acid. The uvr gene of strain Bs3 is unusual in increasing the tendency to filament and also sensitivity to nalidixic acid.

Strains Bs1, Bs3 and Bs8 are all doubly mutated from strain B, the second mutation (not uvr) being responsible for their increased resistance to nalidixic acid as well as partially or completely suppressing filamentation.

It is concluded that the cell division mechanism of (lon) strain B is sensitive to inhibition of DNA synthesis. Mutations which suppress the tendency of strain B to filament reduce its sensitivity to inhibition of DNA synthesis.

Type
Research Article
Information
Genetics Research , Volume 14 , Issue 2 , October 1969 , pp. 111 - 119
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Cummings, D. J. & Mondale, L. (1967). Thymineless death in Escherichia coli: strain specificity. J. Bact. 93, 1917–1924.CrossRefGoogle ScholarPubMed
Cummings, D. J. & Taylor, A. L. (1966). Thymineless death and its relation to UV sensitivity in Escherichia coli. Proc. natn. Acad. Sci. U.S.A. 56, 171–176.CrossRefGoogle ScholarPubMed
Demerec, M., Adelberg, E. A., Clark, A. J. & Hartman, P. E. (1966). A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76.CrossRefGoogle ScholarPubMed
Donch, J., Chung, Y. S. & Greenberg, J. (1969). Locus for radiation resistance in Escherichia coli strain B/r. Genetics (in the Press).CrossRefGoogle Scholar
Donch, J., Green, M. H. L. & Greenberg, J. (1968). Interaction of the exr and lon genes in Escherichia coli. J. Bact. 96, 1704–1710.CrossRefGoogle ScholarPubMed
Donch, J. & Greenberg, J. (1968 a). Ultraviolet sensitivity gene of Escherichia coli B. J. Baci. 95, 1555–1559.CrossRefGoogle ScholarPubMed
Donch, J. & Greenberg, J. (1968 b). Loci of radiation sensitivity in Bs strains of Escherichia coli. Genet. Res., Camb. 11, 183–191.CrossRefGoogle Scholar
Donch, J. & Greenberg, J. (1968 c). Genetic studies of strain Bs8 of Escherichia coli. Genet.Res., Camb. 12, 55–63.CrossRefGoogle ScholarPubMed
Goss, W. A., Deitz, W. H. & Cook, T. M. (1965). Mechanism of action of nalidixic acid on Escherichia coli. II. Inhibition of deoxyribonucleic acid synthesis. J. Bact. 89, 1068–1074.CrossRefGoogle ScholarPubMed
Greenberg, J. (1967). Loci for radiation sensitivity in Escherichia coli strain B5−1. Genetics 55, 193–201.CrossRefGoogle Scholar
Hill, R. F. & Feiner, R. R. (1964). Further studies of ultraviolet-sensitive mutants of Escherichia coli strain B. J. gen. Microbiol. 35, 105–114.CrossRefGoogle ScholarPubMed
Howard-Flanders, P., Boyce, R. P. & Theriot, L. (1966). Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53, 1119–1136.CrossRefGoogle ScholarPubMed
Mattern, I. E., Zwenk, H. & Rörsch, A. (1966). The genetic constitution of the radiation-sensitive mutant Escherichia coli Bs−1. Mutation Res. 3, 374–380.CrossRefGoogle ScholarPubMed
Van De Putte, P., Van Sluis, C. A., Van Dillewijn, J. & Rörsch, A. (1965). The location of genes controlling radiation sensitivity in Escherichia coli. Mutation Res. 2, 97–110.CrossRefGoogle ScholarPubMed