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Effect of inhibition of DNA synthesis on mating in Escherichia coli K12

Published online by Cambridge University Press:  14 April 2009

Susan Hollom  and
R. H. Pritchard
Susan Hollom
Affiliation:
Department of Genetics, The University, Leicester
R. H. Pritchard
Affiliation:
Department of Genetics, The University, Leicester

Extract

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From studies involving inhibition of DNA synthesis in Hfr strains of Escherichia coli K12, either by thymine starvation (Pritchard, 1963) or amino-acid starvation (Suit, Matney, Doudney & Billen, 1964), during mating with F− strains, it has been concluded that transfer of DNA from males to females can occur in the absence of DNA synthesis. This conclusion is at variance with the hypothesis (Jacob, Brenner & Cuzin, 1963) that the energy required for transfer is derived from the process of DNA replication. On the other hand, a second prediction from this hypothesis, that one polynucleotide chain of the DNA transferred during mating will have been synthesized during transfer, is strongly supported by recent experiments (Ptashne, 1965; Blinkova, Bresler & Lanzov, 1965; Gross & Caro, 1965).

Type
Short Notes
Information
Genetics Research , Volume 6 , Issue 3 , November 1965 , pp. 479 - 483
Copyright
Copyright © Cambridge University Press 1965

References

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